Mother's Own Milk

Mother’s own milk is breast milk produced by an infant’s biological mother and provided directly at the breast or as expressed milk, making it an important nutritional and biologically active resource in medicine. Its composition includes nutrients, immunoglobulins, immune cells, hormones, and growth factors that support infant development while helping protect the gastrointestinal and respiratory systems from infection. Milk composition changes across lactation and can adapt to an infant’s developmental needs. In neonatal care, particularly for preterm or medically fragile infants, mother’s own milk supports feeding, growth, and clinical recovery, while informing practices in lactation support, milk expression, storage, and human milk-based care.

Mother's Own Milk - Related Videos

Research

JoVE Journal - Biology

Milk Collection in the Rat Using Capillary Tubes and Estimation of Milk Fat Content by Creamatocrit

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Cited by 24 •

2015

Milk is a primary source of nutrition for the neonate. Analysis of milk components may provide insight into maternal factors that affect offspring health. This protocol describes a manual method of collecting milk samples from the lactating rat, which can then be used for further downstream analysis.

Milk Collection Methods for Mice and Reeves' Muntjac Deer

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Cited by 26 •

2014

Milk collection from animal models facilitates various research avenues: understanding passive immunity, identifying pathogens responsible for vertical transmission and, through the use of transgenic mice, even commercial production of proteins found in human breast milk. Here we illustrate a simple method for milk collection in mice and Reeves’ muntjac deer.

Research

JoVE Journal - Biology
Free Sample

Rapid Isolation of Human Breast Milk-Derived Extracellular Vesicles

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2025

This protocol is designed to expedite and allow for the effective isolation of extracellular vesicles from human breast milk with high purity.

Determination of Sialic Acids in Liver and Milk Samples of Wild-type and CMAH Knock-out Mice.

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Cited by 7 •

2017

We describe a HPLC-based method for the determination of N-acetylneuraminic acid and N-glycolylenuraminic acid in mouse liver and milk.

A Method for Targeted 16S Sequencing of Human Milk Samples

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Cited by 5 •

2018

A semi-automated workflow is presented for targeted sequencing of 16S rRNA from human milk and other low-biomass sample types.

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