Murine Cardiac Transplantation
Murine Cardiac Transplantation - Related Videos
10:27
Murine Heterotopic Heart Transplant Technique
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Cited by 19 •
2014
The manuscript describes the steps required to perform the heterotopic heart transplant in the mouse.
07:59
Murine Full-thickness Skin Transplantation
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Cited by 41 •
2017
Murine full-thickness skin transplantation is a well-established model to study rejection in an alloimmune setting. Here, we provide a tutorial of each step involved in performing a BALB/c-->C57BL/6 full-thickness skin transplant.
10:32
Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique
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Cited by 26 •
2014
The murine cervical heart transplantation model is well suited for immunological as well as ischemia reperfusion injury studies. We modified the procedure using a non-suture cuff technique and performed more than 1,000 successful transplants with this approach. Herein, we provide additional details of this technique to supplement the video.
08:58
Murine Kidney Transplant Technique
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Cited by 8 •
2015
The goal of this manuscript is to describe the steps required to perform a kidney transplant in a mouse, paying particular attention to the details of the arterial anastomosis.
06:42
Murine Skin Transplantation
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Cited by 36 •
2008
Allogeneic skin transplantation is a standard model to assay host T cell responses to MHC-disparate donor antigens. This video-article provides a visual tutorial of each step involved in performing a BALB/c-->C57BL/6 skin transplant.
FAQs
The heterotopic arrangement keeps the recipient’s native heart circulating blood while the donor graft is perfused through its connections to recipient vessels. This separation lets investigators follow graft contractions and survival as transplantation outcomes without making the implanted heart the recipient’s primary pump, supporting controlled study of graft-specific biology.
Murine cardiac transplantation can be used to examine rejection, ischemia-reperfusion injury, inflammation, and immune tolerance as related but distinct transplantation processes. Researchers combine observations of graft function and survival with clinical and tissue-based analyses, allowing functional changes to be considered alongside structural or inflammatory findings rather than relying on a single endpoint.
Monitoring graft contractions provides a direct functional readout during follow-up, while graft survival supplies a longer-term outcome measure. Interpreting these observations together with tissue-based analyses helps investigators connect loss or persistence of function with processes such as rejection, inflammation, or ischemia-reperfusion injury, strengthening mechanistic assessment of the transplant response.
Surgeons connect the donor aorta and pulmonary artery to recipient vessels, creating the vascular arrangement required for graft perfusion. In the heterotopic model, these connections support the implanted heart while the recipient’s native heart continues circulating blood. This setup makes graft performance observable without replacing native circulation.
Because the model offers controlled assessment of graft outcomes, it supports testing immunosuppressive therapies as well as genetic or cellular interventions. Investigators can compare how these approaches affect graft contractions, survival, rejection, inflammation, ischemia-reperfusion injury, or immune tolerance. The resulting observations provide mechanistic insight into how an intervention changes transplantation biology.
In medicine, the model links experimentally controlled transplantation studies with questions relevant to human heart transplantation and cardiovascular medicine. Its value lies in combining a living graft, measurable function, survival follow-up, and tissue-based assessment in one system. That combination helps researchers investigate immune and injury pathways and examine their relevance to clinical transplantation.