Murine Transplant Model
Murine Transplant Model - Related Videos
07:59
Murine Full-thickness Skin Transplantation
0 Views •
Cited by 41 •
2017
Murine full-thickness skin transplantation is a well-established model to study rejection in an alloimmune setting. Here, we provide a tutorial of each step involved in performing a BALB/c-->C57BL/6 full-thickness skin transplant.
10:32
Murine Cervical Heart Transplantation Model Using a Modified Cuff Technique
0 Views •
Cited by 26 •
2014
The murine cervical heart transplantation model is well suited for immunological as well as ischemia reperfusion injury studies. We modified the procedure using a non-suture cuff technique and performed more than 1,000 successful transplants with this approach. Herein, we provide additional details of this technique to supplement the video.
06:42
Murine Skin Transplantation
0 Views •
Cited by 36 •
2008
Allogeneic skin transplantation is a standard model to assay host T cell responses to MHC-disparate donor antigens. This video-article provides a visual tutorial of each step involved in performing a BALB/c-->C57BL/6 skin transplant.
10:27
Murine Heterotopic Heart Transplant Technique
0 Views •
Cited by 19 •
2014
The manuscript describes the steps required to perform the heterotopic heart transplant in the mouse.
08:58
Murine Kidney Transplant Technique
0 Views •
Cited by 8 •
2015
The goal of this manuscript is to describe the steps required to perform a kidney transplant in a mouse, paying particular attention to the details of the arterial anastomosis.
FAQs
Compatibility influences how the recipient mouse recognizes transplanted material and whether the graft becomes tolerated or rejected. The same model can therefore reveal immune recognition, rejection, tolerance, or graft-versus-host responses, depending on the relationship between donor and recipient. Selecting compatibility conditions allows researchers to examine distinct host responses rather than treating transplantation as a single biological process.
These designs represent different donor-recipient relationships and consequently model different interactions with the host environment. Syngeneic, allogeneic, and xenogeneic arrangements can be selected according to whether the study emphasizes compatible engraftment, immune differences between donors and recipients, or broader cross-species transplant responses. This comparison helps investigators connect transplant outcomes with the biological source of the graft.
A transplanted graft must interact with the recipient environment through more than one process. Vascular integration reflects how transplanted material connects with host conditions, while immune recognition determines whether the recipient accepts or attacks it. Monitoring both provides a broader interpretation of engraftment and biological effect, helping distinguish successful incorporation from outcomes dominated by rejection or other host responses.
The workflow begins by selecting donor material, recipient mice, and a compatibility design suited to the scientific question. Researchers then place the cells, tissue, or organ into the recipient and monitor engraftment and biological effects. Assessment may include vascular integration, immune recognition, rejection, tolerance, or graft-versus-host responses, with the selected outcomes reflecting the model’s intended purpose.
A murine transplant model is useful when investigators need to examine how transplanted material behaves in a living host before clinical investigation. Applications described for these systems include testing transplantation strategies, immunosuppressive therapies, regenerative approaches, treatment safety, and tumor biology. The model links the intervention to measurable host responses and can support comparison of alternative experimental designs.
These studies can show whether transplanted material engrafts, integrates with the host environment, or triggers harmful immune responses. They also allow investigators to examine tolerance, rejection, and graft-versus-host effects in relation to donor-recipient compatibility. Such findings provide biological and safety context for transplantation, cancer-related research, regenerative medicine, and therapies considered for later clinical investigation.