Self-reported Adherence
Self-reported Adherence - Related Videos
07:19
Identifying Targets of Human microRNAs with the LightSwitch Luciferase Assay System using 3'UTR-reporter Constructs and a microRNA Mimic in Adherent Cells
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Cited by 22 •
2011
MicroRNAs (miRNAs) are important regulators of gene expression and have been shown to play a role in numerous biological processes. To better understand miRNA-UTR interactions, we have created a genome-wide collection of 3 UTR luciferase reporters paired with a novel luciferase gene and assay reagent, the LightSwitch system.
09:20
In situ Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells
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Cited by 29 •
2010
In situ subcellular fractionation of mammalian cells on microscope coverslips allows the visualisation of protein localisation.
02:59
Bead Loading to Introduce Nucleic Acids Into Adherent Cultured Cells: A Technique to Load Fluorescent Protein-Encoding Plasmids Into Adherent Mammalian Cells
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2025
In this video, we describe the procedure to introduce nucleic acids into adherent mammalian cells using bead loading.
05:30
Clonogenic Assay: Adherent Cells
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Cited by 228 •
2011
The applicability of the clonogenic assay for evaluating reproductive viability has been established for more than 50 years. Here we demonstrate the general procedure for performing the clonogenic assay with adherent cells.
08:21
Permeabilization of Adhered Cells Using an Inert Gas Jet
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Cited by 3 •
2013
This protocol describes a method for the temporary permeabilization of adherent cells using an inert gas jet. This technique facilitates the transfer of genetic material and biomolecules into adherent mammalian cells by the utilization of mechanical forces to disrupt the plasma membrane.
FAQs
Recall difficulties can make patients report medication use, appointments, or lifestyle behaviors inaccurately, particularly when reports depend on remembering past actions. Socially desirable responses may lead patients to describe behavior in a way they believe clinicians or researchers will view favorably. These influences can affect interpretation, so reported adherence should be considered alongside other available evidence.
The approach can address several prescribed behaviors rather than medication use alone. Questionnaires, interviews, diaries, and medication-taking reports may ask about taking treatments, attending appointments, or following lifestyle instructions. Selecting the reporting format around the behavior of interest helps clinicians or researchers examine the specific recommendation they need to evaluate.
Different measurement sources provide complementary information about treatment use. A patient report can describe adherence behavior from the patient’s perspective, while pharmacy records, electronic monitoring, or biological measures offer additional evidence. Comparing these sources can provide a more complete assessment than relying on one method alone and can help researchers or clinicians interpret apparent differences in reported treatment use.
Reports can help clinicians identify difficulties connected with carrying out prescribed recommendations, even when the underlying behavior is not directly observable. This information supports conversations about the patient’s experience and can guide tailored counseling or support. The value lies not only in estimating consistency, but also in using the reported information to inform individualized care decisions.
A basic workflow is to select a reporting format, such as a questionnaire, interview, diary, or medication-taking report, and direct it toward the prescribed behavior of interest. The resulting information can then be interpreted with awareness of recall and response biases. When available, clinicians or researchers can compare it with pharmacy, electronic, or biological measures.
Clinical studies can use patient reports to assess how consistently participants follow recommendations, while healthcare evaluations can examine treatment use across care settings or programs. The method is especially informative when researchers need patient-reported information about medication taking, appointments, or lifestyle instructions. Pairing it with other measures strengthens the overall assessment of adherence.
In medicine, adherence information helps connect a treatment outcome with the extent to which prescribed recommendations were followed. Clinicians can use reported patterns to interpret outcomes, identify possible barriers, and tailor counseling or support. Researchers can similarly use the information when evaluating treatment use, provided they recognize that self-reports may not perfectly reflect actual behavior.