Vascular Biology Techniques
Vascular Biology Techniques - Related Videos
11:22
Engineering Biological-Based Vascular Grafts Using a Pulsatile Bioreactor
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Cited by 32 •
2011
Our group has developed a bioreactor culture system that mimics the physiological pulsatile stresses of the cardiovascular system to regenerate implantable small-diameter vascular grafts.
11:17
Surgical Technique for the Implantation of Tissue Engineered Vascular Grafts and Subsequent In Vivo Monitoring
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Cited by 9 •
2015
A step-by-step protocol for the inter-positional placement of Tissue Engineered Vessels (TEVs) into the carotid artery of a sheep using end-to-end anastomosis and real-time digital assessment in vivo until animal sacrifice.
12:41
A Novel Mammary Fat Pad Transplantation Technique to Visualize the Vessel Generation of Vascular Endothelial Stem Cells
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Cited by 1 •
2017
This work demonstrates a novel approach to assess the proliferation, differentiation, and vessel-forming potential of vascular endothelial stem cells (VESCs) through mammary fat pad transplantation followed by whole-mount tissue preparation for microscopic observation. A lineage tracing strategy to investigate the behavior of VESCs in vivo is also presented.
12:03
Microfluidic Genipin Deposition Technique for Extended Culture of Micropatterned Vascular Muscular Thin Films
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Cited by 4 •
2015
We present a method for microfluidic deposition of patterned genipin and fibronectin on PDMS substrates, allowing extended viability of vascular smooth muscle cell-dense tissues. This tissue fabrication method is combined with previous vascular muscular thin film technology to measure vascular contractility over disease-relevant time courses.
08:57
A Novel Technique for Generating and Observing Chemiluminescence in a Biological Setting
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2017
This protocol describes a new intraoperative imaging technique that uses a ruthenium complex as a source of chemiluminescent light emission, thereby producing high signal-to-noise ratios during in vivo imaging. Intraoperative imaging is an expanding field that could revolutionize the way that surgical procedures are performed.
FAQs
Shear stress provides a flow-related condition for examining how blood movement alters vascular behavior. Flow-based assays can expose vascular systems to this mechanical stimulus and reveal changes associated with endothelial function, signaling, inflammation, and remodeling. Including it helps researchers connect molecular or cellular responses with physiologically relevant blood-flow conditions.
Fluorescence microscopy helps researchers examine vascular structure and locate changes within cells or vessel models. When paired with endothelial cell culture or molecular measurements, it can connect visible alterations with signaling activity, inflammatory stimulation, permeability, or remodeling. This combination supports interpretation of how cellular changes may affect vessel behavior.
Endothelial cell culture provides a controlled system for testing how vascular cells respond to signaling pathways or inflammatory stimuli. Gene and protein analysis then measures molecular changes associated with those responses. Together, these approaches help relate altered molecular activity to endothelial dysfunction, permeability, angiogenesis, or other vascular outcomes relevant to disease research.
Flow-based assays add information about how vascular behavior changes under blood-flow conditions, including the influence of shear stress. Gene or protein analysis can identify accompanying molecular changes, while microscopy can examine structural effects. Using these readouts together helps link signaling and inflammation with permeability, remodeling, and functional vascular responses.
A study can begin by selecting an endothelial cell or vessel model, then applying a relevant condition such as flow or an inflammatory stimulus. Researchers may combine fluorescence microscopy with gene, protein, or flow-based measurements to assess structure, signaling, and function. The resulting comparison links experimental conditions with vascular outcomes.
These methods are useful when researchers need to model vascular disease, investigate angiogenesis or thrombosis, identify biomarkers, or evaluate cardiovascular and cancer therapies. They can show how treatment or disease-related stimuli affect endothelial behavior, permeability, remodeling, and blood flow. This evidence supports drug development and more precise treatment strategies.