Agarose Embedded Slice
Agarose Embedded Slice - Related Videos
02:59
Agarose-Embedded Electroporation-Assisted Gene Transfer in Mouse Cortical Interneuron Progenitors
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2025
Source: Denaxa, M., el al., Transplantation of Chemogenetically Engineered Cortical Interneuron Progenitors into Early Postnatal Mouse Brains. J. Vis. Exp. (2019)This video demonstrates the procedure for injecting a plasmid vector carrying the gene of interest into interneuron progenitors in an embryonic brain slice, followed by electroporation for plasmid entry and gene expression. These modified progenitors can later be used for treating brain disorders.
04:26
Agarose-Based Preparation of Human Precision-Cut Lung Slices
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2025
In this video, we describe a procedure to inflate isolated human lungs with agarose and prepare human precision-cut lung slices. The prepared ex vivo lung slices can be used for further immunologic experiments.
02:59
Embedding 3D Spheroids in Agarose Gel Drops: A Technique to Preserve Spheroids for Downstream Analysis
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2023
This video describes the technique to embed 3D spheroids in agarose gel drops for preservation. The embedded 3D spheroids can be further used for downstream analysis.
13:50
Post-embedding Immunogold Labeling of Synaptic Proteins in Hippocampal Slice Cultures
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Cited by 13 •
2013
The localization and distribution of proteins provide important information for understanding their cellular functions. The superior spatial resolution of electron microscopy (EM) can be used to determine the subcellular localization of a given antigen following immunohistochemistry. For tissues of the central nervous system (CNS), preserving structural integrity while maintaining antigenicity has been especially difficult in EM studies. Here, we adopt a procedure that has been used to preserve...
07:17
Automated Vibratome Sectioning of Agarose-Embedded Lung Tissue for Multiplex Fluorescence Imaging
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Cited by 5 •
2023
We have developed a tissue-processing technique utilizing a vibratome and agarose-embedded lung tissue to generate lung sections, thereby permitting the acquisition of high-resolution images of lung architecture. We employed immunofluorescence staining to observe spatial protein expression using specific lung structural markers.
FAQs
Once the agarose solidifies around the nervous tissue, it provides mechanical support during blade passage. The vibratome or similar instrument moves the blade through both matrix and specimen under controlled conditions, limiting movement and deformation. This support helps preserve section integrity, making the resulting slices more suitable for microscopic and experimental analysis.
Limiting movement helps maintain the structural relationships within the specimen while it is being sectioned. Preserved tissue organization supports more consistent examination of neural circuits, cellular morphology, and broader anatomical features. In practice, improved stability also makes slices easier to handle for downstream microscopic analyses and comparisons between preparations.
Solidification creates a supportive material surrounding the specimen before the blade advances through it. The resulting matrix-tissue combination can be sectioned as a more stable unit than unsupported tissue alone, reducing deformation during cutting. This step is therefore central to producing sections with sufficient integrity for neuroanatomical and cellular studies.
The method combines matrix support with controlled blade advancement, improving the stability and handling of sections. More consistent section integrity can facilitate examination across samples, whether the goal is to map neuroanatomical organization, assess cellular morphology, or identify structural changes associated with disease-related tissue conditions.
The workflow begins by enclosing the brain, spinal cord, or other nervous tissue in agarose. After the matrix solidifies around the specimen, the embedded preparation is positioned for sectioning. A vibratome or similar instrument then advances a blade through the agarose and tissue under controlled conditions to generate stable slices.
Researchers may select this preparation when stable brain or spinal cord sections are needed for microscopic and experimental analysis. Supported slices can be used in neuroanatomical mapping, immunohistochemistry, and cellular morphology studies. The approach is especially relevant when preserving tissue integrity and handling quality is important for examining neural organization.
Prepared sections can support examination of neural circuits, tissue organization, and cellular morphology. They also provide material for studying disease-related structural changes through microscopic analysis and immunohistochemistry. Because the matrix helps maintain section integrity, observations can be made on tissue architecture with less concern that cutting-related deformation obscured relevant features.