Heat Gradient Device

A heat gradient device is an experimental system that creates controlled differences in temperature across a defined surface or space, enabling precise study of thermal sensing and behavior. It works by regulating heating elements or temperature-controlled regions to establish stable or changing thermal gradients, while sensors monitor the delivered temperature and experimental conditions. In neuroscience, these devices help researchers examine thermoreception, nociception, sensory coding, and neural responses to warming or cooling stimuli. By linking controlled thermal input with behavioral, physiological, or neural measurements, heat gradient devices support investigations of sensory processing and may inform research on pain, temperature perception, and neurological disorders.

Heat Gradient Device - Related Videos

Research

JoVE Journal - Biology

A Gradient-generating Microfluidic Device for Cell Biology

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Cited by 14 •

2007

We describe a protocol for the microfabrication of the gradient-generating microfluidic device that can generate spatial and temporal gradients in well-defined microenvironment. In this approach, the gradient-generating microfluidic device can be used to study directed cell migration, embryogenesis, wound healing, and cancer metastasis.

A Microfluidic Device for Quantifying Bacterial Chemotaxis in Stable Concentration Gradients

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Cited by 7 •

2010

This protocol describes the development of a microfluidic device for investigating bacterial chemotaxis in stable concentration gradients of chemoeffectors.

Research

JoVE Journal - Bioengineering
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Polydimethylsiloxane-polycarbonate Microfluidic Devices for Cell Migration Studies Under Perpendicular Chemical and Oxygen Gradients

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Cited by 13 •

2017

The control of chemical and oxygen gradients is essential for cell cultures. This paper reports a polydimethylsiloxane-polycarbonate (PDMS-PC) microfluidic device capable of reliably generating combinations of chemical and oxygen gradients for cell migration studies, which can be practically utilized in biological labs without sophisticated instrumentation.

Thermal Measurement Techniques in Analytical Microfluidic Devices

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Cited by 6 •

2015

Here, we present three protocols for thermal measurements in microfluidic devices.

Education

JoVE Science Education - Chemistry

Density Gradient Ultracentrifugation

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2023

Density gradient ultracentrifugation is a common technique used to isolate and purify biomolecules and cell structures. This technique exploits the fact that, in suspension, particles that are more dense than the solvent will sediment, while those that are less dense will float. A high-speed ultracentrifuge is used to accelerate this process in order to separate biomolecules within a density gradient, which can be established by layering liquids of decreasing density in a centrifuge tube. The...

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