Laser-induced Bbb Disruption
Laser-induced Bbb Disruption - Related Videos
01:26
Laser-Induced Blood-Brain Barrier Disruption for Liposome Delivery into a Mouse Model of Glioblastoma
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2025
Begin with a mouse model of glioblastoma, an invasive brain tumor.At the tumor periphery, the blood-brain barrier (BBB) remains intact, with endothelial cells forming tight junctions that regulate molecular entry.The mouse is fitted with a head-mounted optical fiber to deliver laser light.Administer a low-intensity infrared laser, which penetrates the skin and skull to reach the brain.The laser excites molecular oxygen in brain cells, generating reactive oxygen species (ROS).The resulting...
01:27
Enhancing Drug Delivery in Recurrent Glioblastoma with Laser-Induced Hyperthermia
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2025
Begin with an anesthetized human patient diagnosed with recurrent glioblastoma, an invasive tumor characterized by tumor cell infiltration into the surrounding peritumoral region.The peritumoral region retains an intact blood-brain barrier (BBB), where tight junctions between endothelial cells restrict drug penetration.Make an incision in the scalp, create an opening in the skull, and insert a laser probe into the tumor core using MRI guidance.Deliver laser light through the probe.Tissue...
16:26
Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport
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Cited by 58 •
2014
The aim of the present study was to validate the reproducibility of an in vitro BBB model involving a rat syngeneic co-culture of endothelial cells and astrocytes. The endothelial cell monolayer presented high TEER and low LY permeability. Expression of specific TJ proteins, functional responses to inflammation and functionality of transporters and receptors were assessed.
03:01
Assessing Blood-Brain Barrier Integrity in a Laser-Induced Brain Injury Rat Model
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2025
Source: Kuts, R. et al. Laser-Induced Brain Injury in the Motor Cortex of Rats. J. Vis. Exp. (2020)This video demonstrates assessing blood-brain barrier (BBB) integrity following laser-induced brain injury in rats. A tracking dye is injected into the tail vein, allowing it to circulate to the brain and leak into the tissue through the damaged BBB. The brain is then extracted and processed to release the dye. The fluorescence intensity of the dye is measured to quantify the extent of...
08:07
Laser-induced Forward Transfer of Ag Nanopaste
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Cited by 13 •
2016
We demonstrate the use of the Laser-induced forward transfer technique (LIFT) for the printing of high-viscosity Ag paste. This technique offers a simple, low temperature, robust process for non-lithographically printing microscale 2D and 3D structures.
FAQs
Focused laser energy produces localized photothermal effects in brain microvascular regions. These effects alter endothelial cells and the tight junctions that normally regulate movement between blood and brain tissue. The resulting change in barrier properties can permit selected molecules or therapeutic agents to cross, while the focused exposure helps limit effects in surrounding tissue.
Laser wavelength, intensity, and exposure duration determine how strongly and how long tissue experiences photothermal effects. Adjusting these variables helps researchers balance increased permeability against unwanted tissue injury. The goal is a controlled, localized, and reversible opening rather than excessive disruption, so parameter selection is central to experimental design and translational development.
Reversibility allows the barrier to open long enough for selected molecules or therapeutic agents to cross without maintaining a lasting change in brain vascular protection. This property supports controlled delivery and reduces the intended exposure of surrounding tissue. Consequently, experiments can investigate transport or treatment strategies while emphasizing protection of normal barrier function.
A controlled experiment must regulate the laser wavelength, intensity, and exposure duration, while concentrating energy on the intended region. These conditions determine the balance between permeability change and tissue injury. Careful control is therefore necessary to produce a localized effect, support reversible opening, and make results more interpretable in neuroscience research.
By locally changing barrier permeability, this technique can help selected therapeutic agents move from the bloodstream into a defined brain region. The focused nature of the exposure may limit effects on surrounding tissue compared with a broadly distributed barrier change. This makes the approach relevant to investigations of more targeted delivery strategies for neurological disease.
In neuroscience, the method supports three major lines of work: targeted drug delivery, molecular imaging, and experimental studies of blood-brain barrier function. Researchers can use controlled permeability changes to examine how selected molecules reach brain tissue, evaluate imaging approaches, or study barrier behavior in the context of neurological disease.