Journal
/
Medicine
/
Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport
JoVE Journal
Medicine
This content is Free Access.
JoVE Journal Medicine
Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport

Setting-up an In Vitro Model of Rat Blood-brain Barrier (BBB): A Focus on BBB Impermeability and Receptor-mediated Transport

DOI:
10.3791/51278-v

•

16:26 min

•

June 28, 2014

•

, , , ,
1VECT-HORUS SAS, 2Aix-Marseille Université,CNRS, NICN UMR 7259

Chapters

  • 00:05Title
  • 02:55Isolation of Rat Brain Microvessels
  • 09:27Purification and Proliferation of RBEC Primocultures
  • 10:32Differentiation: Setting up the In Vitro BBB Model
  • 14:50Results: Immunocytochemical Characterization of the Rat In Vitro BBB Model and the Paracellular Permeability of LY across the Endothelial Monolayer
  • 15:44Conclusion

Summary

Automatic Translation

Automatic Translation

The aim of the present study was to validate the reproducibility of an in vitro BBB model involving a rat syngeneic co-culture of endothelial cells and astrocytes. The endothelial cell monolayer presented high TEER and low LY permeability. Expression of specific TJ proteins, functional responses to inflammation and functionality of transporters and receptors were assessed.

Tags

Keywords: In VitroRatBlood-brain BarrierPrimary Brain Endothelial CellsAstrocytesCo-cultureTight JunctionsTransendothelial Electrical ResistancePermeabilityP-glycoproteinTranscytosisReceptor-mediated Transport

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/51278?article_type=v

MRI-guided Disruption of the Blood-brain Barrier using Transcranial Focused Ultrasound in a Rat Model
check_url/51278?article_type=v

Rat Model of Blood-brain Barrier Disruption to Allow Targeted Neurovascular Therapeutics
check_url/51278?article_type=v

Stretch in Brain Microvascular Endothelial Cells (cEND) as an In Vitro Traumatic Brain Injury Model of the Blood Brain Barrier
check_url/51278?article_type=v

Excitotoxic Stimulation of Brain Microslices as an In vitro Model of Stroke
check_url/51278?article_type=v

Oxygen-Glucose Deprivation and Reoxygenation as an In Vitro Ischemia-Reperfusion Injury Model for Studying Blood-Brain Barrier Dysfunction
check_url/51278?article_type=v

In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption
check_url/51278?article_type=v

Improved Method for the Establishment of an In Vitro Blood-Brain Barrier Model Based on Porcine Brain Endothelial Cells
check_url/51278?article_type=v

Endothelial Cell Transcytosis Assay as an In Vitro Model to Evaluate Inner Blood-Retinal Barrier Permeability
check_url/51278?article_type=v

Advancing Hepatic Fibrosis Diagnosis Using Magnetic Resonance Elastography and AI (Video) | JoVE
check_url/51278?article_type=v

Author Spotlight: Modeling an Aspect of Preeclampsia in Female Mice Using Hypoxic Human Placenta-Derived Small Extracellular Vesicles

Read Article