Slice Electrophysiology
Slice Electrophysiology - Related Videos
02:14
Electrophysiological Recordings of Reprogrammed Neurons in a Mouse Brain Slice
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2025
This video demonstrates a protocol to evaluate the maturation and reprogramming of glial cells into neurons in a transfected mouse striatum slice. The experiment measures resting membrane potential and response to current injections using neuron-specific fluorescent markers and electrophysiological techniques. Voltage spikes during these measurements indicate successful neuronal maturation and reprogramming.
07:51
Murine Short Axis Ventricular Heart Slices for Electrophysiological Studies
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Cited by 3 •
2017
Here, we describe the preparation of viable ventricular slices from adult mice and their use for sharp electrode action potential recordings. These multicellular preparations provide a preserved in vivo like tissue structure, which makes them a valuable model for electrophysiological and pharmacological studies in vitro.
02:42
Establishing a Whole-Cell Voltage-Clamp Configuration for Electrophysiological Recordings in Brain Slices
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2025
This video demonstrates positioning a fine-pointed pipette near brain slices, forming a seal with a neuronal cell. Suction breaks the cell membrane, thereby creating a whole-cell patch clamp that enables current measurements for electrophysiology experiments.
16:38
Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices
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Cited by 19 •
2012
The preparation of acute brain slices from isolated hippocampi, as well as the simultaneous electrophysiological recordings of astrocytes and neurons in stratum radiatum during stimulation of schaffer collaterals is described. The pharmacological isolation of astroglial potassium and glutamate transporter currents is demonstrated.
04:08
Simultaneous Optical and Electrophysiological Monitoring of Neuronal Cells in Brain Slices
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2025
This video outlines a method for simultaneously imaging and recording electrical activity from transgenic brain slices that contain neuron-specific fluorescent proteins. The procedure involves maintaining the brain slice in a perfusion chamber with aerated artificial cerebrospinal fluid, using a light source to excite fluorescent-labeled cells, and recording the electrical signals with a multichannel probe and patch clamp.
FAQs
Preserving local connections lets investigators examine how neurons influence one another within a circuit rather than measuring only the properties of isolated cells. This distinction supports analysis of synaptic transmission, circuit responses, and plasticity, meaning activity-related changes in synaptic function. As a result, the method connects cellular electrical measurements with more integrated questions about neural network function.
Whole-cell patch-clamp and extracellular recordings provide complementary ways to measure electrical behavior. The measurements may include membrane potentials, synaptic currents, or responses of circuits to stimulation. Using one configuration or the other helps align the recording with the biological question, whether the focus is an individual neuron's intrinsic excitability, communication between neurons, or broader circuit activity.
Keeping the slice in oxygenated artificial cerebrospinal fluid supports the living tissue during recording, while controlled stimulation or pharmacological agents let researchers test how activity changes under defined conditions. These manipulations make it possible to compare electrical behavior with responses to specific inputs or compounds, helping distinguish intrinsic neuronal properties from synaptic or circuit-level effects.
The workflow combines a living thin tissue section, oxygenated artificial cerebrospinal fluid, and an electrode-based recording arrangement. Researchers position the slice in the fluid, use whole-cell patch-clamp or extracellular electrodes, and then record electrical responses while applying controlled stimuli or pharmacological agents. This sequence links experimental conditions to measurements of membrane, synaptic, or circuit activity.
Slice electrophysiology is useful when a study must connect cellular signals with preserved local circuitry. In neuroscience, recordings can examine synaptic transmission, intrinsic excitability, plasticity, and network function. Those readouts support investigations of learning, sensory processing, neurodevelopment, disease mechanisms, and potential neurological therapies, allowing researchers to test electrical consequences within a controlled tissue preparation.
Because the preparation retains local cellular connections better than isolated-cell preparations, it provides access to both neuronal properties and interactions within nearby networks. Researchers can examine whether a change reflects a neuron's own excitability, altered synaptic communication, or a broader circuit response. This layered view is especially relevant when interpreting plasticity or disease-related changes in neuroscience.