Journal
/
Neuroscience
/
Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices
JoVE Journal
Neuroscience
Author Produced
This content is Free Access.
JoVE Journal Neuroscience
Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

Dual Electrophysiological Recordings of Synaptically-evoked Astroglial and Neuronal Responses in Acute Hippocampal Slices

DOI:
10.3791/4418-v

•

16:38 min

•

November 26, 2012

•

, ,
1Neuroglial Interactions in Cerebral Physiopathology, Center for Interdisciplinary Research in Biology, CNRS UMR 7241, INSERM U1050,Collège de France, 2Paris Diderot University

Chapters

  • 00:00Title
  • 03:58Dissection
  • 10:23Dual Recordings of Neuronal fEPSP and Single Astrocytic Whole-cell Response
  • 12:07Dual Whole-cell Recordings of Astrocyte and Neuron
  • 13:53Representative Results
  • 15:45Conclusion

Summary

Automatic Translation

Automatic Translation

The preparation of acute brain slices from isolated hippocampi, as well as the simultaneous electrophysiological recordings of astrocytes and neurons in stratum radiatum during stimulation of schaffer collaterals is described. The pharmacological isolation of astroglial potassium and glutamate transporter currents is demonstrated.

Tags

Dual Electrophysiological RecordingsSynaptically-evoked Astroglial ResponsesNeuronal ResponsesAcute Hippocampal SlicesTripartite SynapsesAstrocytesNeuronal Activity ModulationIon ChannelsNeurotransmitter ReceptorsGliotransmittersGlutamate UptakePotassium Spatial BufferingGABA ClearanceSynaptic Activity MonitoringAlterations In Glutamate LevelsAlterations In GABA LevelsAlterations In Extracellular Potassium LevelsAstroglial Uptake ActivityBuffering CapacityPhysiopathological SituationsKnockout MiceHippocampal Slice PreparationStratum Radiatum Astrocytes IdentificationSimultaneous Neuronal And Astroglial RecordingsSynaptically-evoked Astroglial Currents Isolation

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/4418?article_type=v

Dual Somatic Recordings from Gonadotropin-Releasing Hormone (GnRH) Neurons Identified by Green Fluorescent Protein (GFP) in Hypothalamic Slices
check_url/4418?article_type=v

Preparation of Acute Hippocampal Slices from Rats and Transgenic Mice for the Study of Synaptic Alterations during Aging and Amyloid Pathology
check_url/4418?article_type=v

Paired Whole Cell Recordings in Organotypic Hippocampal Slices
check_url/4418?article_type=v

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings
check_url/4418?article_type=v

Investigation of Synaptic Tagging/Capture and Cross-capture using Acute Hippocampal Slices from Rodents
check_url/4418?article_type=v

Recording Synaptic Plasticity in Acute Hippocampal Slices Maintained in a Small-volume Recycling-, Perfusion-, and Submersion-type Chamber System
check_url/4418?article_type=v

Acute In Vivo Electrophysiological Recordings of Local Field Potentials and Multi-unit Activity from the Hyperdirect Pathway in Anesthetized Rats
check_url/4418?article_type=v

Evaluation of Synapse Density in Hippocampal Rodent Brain Slices
check_url/4418?article_type=v

Optogenetic Entrainment of Hippocampal Theta Oscillations in Behaving Mice
check_url/4418?article_type=v

Preparation of Acute Human Hippocampal Slices for Electrophysiological Recordings

Read Article