Transient Receptor Potential Channels
Transient Receptor Potential Channels - Related Videos
08:39
Purification of Endogenous Drosophila Transient Receptor Potential Channels
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Cited by 9 •
2021
Based on the assembling mechanism of the INAD protein complex, in this protocol, a modified affinity purification plus competition strategy was developed to purify the endogenous Drosophila TRP channel.
10:00
Controllable Ion Channel Expression through Inducible Transient Transfection
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Cited by 2 •
2017
Studying ion channels through a heterologously expressing system has become a core technique in biomedical research. In this manuscript, we present a time efficient method to achieve tightly controlled ion channel expression by performing transient transfection under the control of an inducible promoter.
08:13
Optical Mapping of Action Potentials and Calcium Transients in the Mouse Heart
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Cited by 71 •
2011
This paper details the dissection procedure, instrumental setup, and experimental conditions during optical mapping of transmembrane potential (Vm) and intracellular calcium transient (CaT) in intact isolated Langendorff perfused mouse hearts.
10:59
Use of Label-free Optical Biosensors to Detect Modulation of Potassium Channels by G-protein Coupled Receptors
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Cited by 5 •
2014
Optical biosensor techniques can detect changes in mass near the plasma membrane in living cells and allow one to follow cellular responses in both individual cells and populations of cells. This protocol will describe detection of the modulation of potassium channels by G-protein coupled receptors in intact cells using this approach.
09:39
Establishing a Mouse Model of a Pure Small Fiber Neuropathy with the Ultrapotent Agonist of Transient Receptor Potential Vanilloid Type 1
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Cited by 6 •
2018
This study establishes an experimental model of pure small fiber neuropathy with resiniferatoxin (RTX). A unique dose of RTX (50 µg/kg) is optimal for developing a small fiber neuropathy model that mimics patient characteristics and could help investigate the nociceptive molecular significance underlying neuropathic pain.
FAQs
Activation opens an ion-conducting pore, allowing cations such as calcium and sodium to enter the neuron. This inward current shifts the membrane toward depolarization, changing how readily the cell responds to subsequent signals. The resulting excitability change links an external or cellular stimulus to neural signaling and helps explain how sensory inputs can influence neuronal activity.
Calcium and sodium both contribute to depolarization, but their entry reflects the channel’s ion-permeation properties and can produce different cellular consequences. TRP channel families vary in ion selectivity, so the relative contribution of each cation is not uniform. Examining this variation helps researchers connect channel properties with distinct patterns of neuronal signaling and sensory transduction.
TRP channels can respond to temperature, mechanical force, lipids, or chemical ligands, indicating that their gates are sensitive to diverse physical and chemical cues. The relevant stimulus determines when a pore becomes permissive to cations. This stimulus-dependent gating allows neurons to distinguish environmental conditions and cellular signals rather than treating every input as the same type of information.
Differences in gating behavior and ion selectivity mean that TRP channel families do not produce identical electrical responses. One family may be influenced by a particular class of stimulus or permit cations with a different balance, altering the resulting depolarization. Comparing these properties helps clarify how molecular channel diversity supports different sensory and cellular functions.
Researchers examine these channels to connect specific stimuli with neuronal responses in pain, itch, thermal perception, and mechanosensation. Studying which signals activate channels and how activation changes membrane excitability helps identify steps in the conversion of sensory information into neural activity. This approach provides a molecular framework for analyzing how distinct sensory modalities are represented in the nervous system.
TRP channel studies can reveal how temperature, force, lipids, and chemical ligands influence neuronal excitability through cation entry. They also show how gating and ion selectivity shape the electrical outcome of stimulation. Together, these observations help researchers distinguish the stimulus conditions associated with particular sensory responses and identify channel properties relevant to pain, itch, and mechanosensation.
Altered TRP channel activity is associated with neurological disease, making channel behavior relevant to disease mechanisms as well as normal sensation. Because these proteins link diverse stimuli to neuronal excitability, changing their activity could influence abnormal sensory or neural signaling. Researchers therefore investigate them as possible intervention points while relating channel properties to specific neurological outcomes.