גידול במוח / Organotypic Slice משותף תרבות מערכת לימוד microenvironment גידול וממוקד תרופות טיפולים

10.9K צפיות

צוטט 29 פעמים

10:13 דק׳

7 בנובמבר 2015

10.3791/53304-v

7 בנובמבר 2015

10.9K צפיות

Emily J. Chadwick¹ , David P. Yang¹ , Mariella G. Filbin² , Emanuele Mazzola³ , Yu Sun¹ , Oded Behar¹^,⁴ , Maria F. Pazyra-Murphy¹ , Liliana Goumnerova⁵ , Keith L. Ligon⁶ , Charles D. Stiles¹ , Rosalind A. Segal¹

¹Department of Cancer Biology, Dana-Farber Cancer Institute, ²Department of Pediatrics, Children's Hospital, ³Department of Biostatistics & Computational Biology, Dana-Farber Cancer Institute, ⁴Department of Developmental Biology and Cancer Research, Hebrew University of Jerusalem, ⁵Department of Neurosurgery, Children's Hospital, ⁶Center for Molecular Oncologic Pathology, Department of Medical Oncology, Dana-Farber Cancer Institute

Many types of human brain tumors are localized to specific regions within the brain and are difficult to grow in culture. This protocol addresses the role of tumor microenvironment and investigates new drug treatments by analyzing fluorescent primary brain tumor cells growing in an organotypic mouse brain slice.