Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

Snezana Vujicic; Lanfei Feng; Angelika Antoni; Joyce Rauch; Jerrold S. Levine

doi:10.3791/54980

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Biology

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זיהוי של איתות תאיים אירועים מושרה תאים קיימא על ידי אינטראקציה עם תאים שכנים מוות של תאים אפופטוטיים שעברו

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

JoVE Journal
Biology

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JoVE Journal Biology

Identification of Intracellular Signaling Events Induced in Viable Cells by Interaction with Neighboring Cells Undergoing Apoptotic Cell Death

זיהוי של איתות תאיים אירועים מושרה תאים קיימא על ידי אינטראקציה עם תאים שכנים מוות של תאים אפופטוטיים שעברו

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8,571 Views

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09:18 min

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December 27, 2016

DOI:

10.3791/54980-v

DOI:

10.3791/54980-v

•

09:18 min

December 27, 2016

•

8561 Views

Snezana Vujicic*^1,2, Lanfei Feng*^1,2, Angelika Antoni³, Joyce Rauch⁴, Jerrold S. Levine^1,2,5

¹Section of Nephrology, Department of Medicine,University of Illinois at Chicago ²Section of Nephrology, Department of Medicine,Jesse Brown Veterans Affairs Medical Center ³Department of Biology,Kutztown University of Pennsylvania ⁴Division of Rheumatology, Department of Medicine,Research Institute of the McGill University Health Centre ⁵Department of Microbiology & Immunology,University of Illinois at Chicago

Summary

Here, we present a protocol for the determination of intracellular signaling events induced in viable cells by physical interaction with adjacent dead or dying cells. The protocol focuses on signaling events induced by receptor-mediated recognition of the dead cells, as opposed to their phagocytic uptake or release of soluble mediators.