7,625 Views
•
06:03 min
•
November 01, 2018
DOI:
Summary
Automatically generated
עכבר הביצים, העוברים בשלב מוקדם מוגנים על-ידי zona pellucida, מטריצה גליקופרוטאין המהווה מחסום נגד המסירה הגן. מאמר זה מתאר עבור לנקב את zona עם לייזר כדי מגלי תאים עובריים עם וקטורים lentiviral וכדי ליצור העכברים הטרנסגניים פרוטוקול.
Related Videos
08:59
Laser-assisted Cytoplasmic Microinjection in Livestock Zygotes
Related Videos
8993 Views
15:01
Windowing Chicken Eggs for Developmental Studies
Related Videos
27925 Views
10:21
Lentiviral Mediated Production of Transgenic Mice: A Simple and Highly Efficient Method for Direct Study of Founders
Related Videos
9589 Views
04:47
Obtaining Eggs from Xenopus laevis Females
Related Videos
14698 Views
05:09
Subretinal Injection of Gene Therapy Vectors and Stem Cells in the Perinatal Mouse Eye
Related Videos
33213 Views
09:39
CRISPR-mediated Loss of Function Analysis in Cerebellar Granule Cells Using In Utero Electroporation-based Gene Transfer
Related Videos
9808 Views
11:48
Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation
Related Videos
15841 Views
10:57
CRISPR/Cas9-Mediated Highly Efficient Gene Targeting in Embryonic Stem Cells for Developing Gene-Manipulated Mouse Models
Related Videos
3856 Views
01:58
Lentiviral Vector-Based Perivitelline Injection: A Method to Transduce the Gene of Interest Into Fertilized Single-Cell Mouse Oocytes
Related Videos
1215 Views
01:49
Laser-Assisted Lentiviral Gene Delivery: A Technique to Permeabilize Mouse Fertilized Eggs to Facilitate Lentiviral Gene Delivery
Related Videos
991 Views
Read Article
Cite this Article
Martin, N. P., Myers, P., Goulding, E., Chen, S., Walker, M., Porter, T. M., Van Gorder, L., Mathew, A., Gruzdev, A., Scappini, E., Romeo, C. Laser-assisted Lentiviral Gene Delivery to Mouse Fertilized Eggs. J. Vis. Exp. (141), e58327, doi:10.3791/58327 (2018).
Copy