Northeast Forestry University 2 articles published in JoVE Biology Protocols for CRISPR/Cas9 Mutagenesis of the Oriental Fruit Fly Bactrocera dorsalis Jinxi Yuan1, Jie Zhang1, Yan Zhang1, WuYun QiQiGe1, Wei Liu2, Shanchun Yan1, Guirong Wang2 1Key Laboratory of Sustainable Forest Ecosystem Management - Ministry of Education, Northeast Forestry University, 2Shenzhen Branch, Guangdong Laboratory of Lingnan Modern Agriculture, Genome Analysis Laboratory of the Ministry of Agriculture and Rural Affairs, Agricultural Genomics Institute at Shenzhen, Chinese Academy of Agricultural Sciences This paper presents the step-by-step protocols for CRISPR/Cas9 mutagenesis of the Oriental fruit fly Bactrocera dorsalis. Detailed steps provided by this standardized protocol will serve as a useful guide for generating mutant flies for functional gene studies in B. dorsalis. Biology Stomata Tape-Peel: An Improved Method for Guard Cell Sample Preparation Sheldon Lawrence II1,2,3, Quiying Pang2,3,4, Wenwen Kong2,3, Sixue Chen1,2,3,5 1Plant Molecular and Cellular Biology Program, University of Florida, 2Department of Biology, University of Florida, 3Genetics Institute (UFGI), University of Florida, 4Alkali Soil Natural Environmental Science Center, Northeast Forestry University, 5Interdisciplinary Center for Biotechnology Research (ICBR), University of Florida This protocol describes a method of preparing enriched stomatal guard cells that is useful for physiological and other biological studies.