Sejong University 1 article published in JoVE Biology Split Green Fluorescent Protein System to Visualize Effectors Delivered from Bacteria During Infection Hye-Young Lee1, So Eui Lee1, Jongchan Woo2, Doil Choi1, Eunsook Park1 1Department of Plant Science, Plant Genomics and Breeding Institute, College of Agriculture and Life Science, Seoul National University, 2Department of Bioindustry and Bioresource Engineering, Sejong University Fluorescent protein-based approaches to monitor effectors secreted by bacteria into host cells are challenging. This is due to the incompatibility between fluorescent proteins and the type-III secretion system. Here, an optimized split superfolder GFP system is used for visualization of effectors secreted by bacteria into the host plant cell.