St. Vincent's Institute of Medical Research View Institution's Website 1 article published in JoVE Biology Extraction of Tissue Antigens for Functional Assays Andra Necula1, Rochna Chand1, Batool Albatat1, Stuart I. Mannering1,2 1Immunology and Diabetes Unit, St. Vincent's Institute of Medical Research, 2Department of Medicine, University of Melbourne A simple protocol for preparing extracts of human tissue to be used as a source of antigens in functional T-cell assays is described. This method allows T-cell responses to tissue-derived antigens to be measured in vitro.
