Name: chromatid movement by microtubule forces
Uploaded: 2021-04-07T12:12:02.000Z
Duration: 1 min 39 s
Channel: JoVE
Description: Chromatid movement during anaphase depends on microtubule forces at the spindle. Kinetochore microtubules grow from the spindle poles, and their plus ends attach to kinetochores on sister chromatids. The kinetochore protein complex Ndc80 makes low-affinity links with the microtubule plus end.

K12 Standards

Next Generation Science Standards

High School

Life Science

from-molecules-to-organisms-structures-and-processes

From Molecules to Organisms: Structures and Processes

HS-LS1-4

Anaphase A and B

La segregazione cromosomica avviene durante l'anafase: quando i cromatidi fratelli si separano e i singoli cromatidi si spostano verso i poli opposti della cellula.
La progressione dell'anafase costituisce due processi indipendenti ma sovrapposti: l'anafase A e l'anafase B.
Durante l'Anafase A, in assenza di coesione fratello-cromatide, due forze verso i poli agiscono sui cromosomi.
La depolimerizzazione dell'estremità positiva dei microtubuli al cinetocore produce una forza verso i poli. Il flusso di microtubuli, dalla depolimerizzazione dell'estremità negativa, genera anche una forza verso i poli. La combinazione di queste due forze verso i poli, accompagnata da un accorciamento dei cinetocorei-microtubuli, tira i singoli cromatidi verso i poli del fuso.
Quando i cromosomi figli si spostano verso i poli, inizia l'anafase B e i poli del fuso vengono separati, allungando il fuso. Le proteine motrici, la chinesina-5 e la dineina, guidano la separazione dei poli-fuso.
Le proteine motrici della chinesina-5 reticolano le estremità positive dei microtubuli interpolari sovrapposti. Queste proteine motorie dirette all'estremità positiva generano una forza all'indietro lungo i microtubuli, allontanando i poli del fuso. 
Le proteine motrici della dineina collegano le estremità dei microtubuli astrali con i componenti della corteccia cellulare. Queste proteine motorie dirette all'estremità negativa generano una forza, tirando i poli del fuso verso la corteccia cellulare.

<ol>
	<li>Alberts, Bruce. "Motori molecolari". Biologia molecolare della cellula. 4a edizione. Biblioteca Nazionale di Medicina degli Stati Uniti, 1 gennaio 1970. [<a href="https://www.ncbi.nlm.nih.gov/books/NBK26888/">Sorgente</a>]</li>
	<li>Joglekar, Ajit P, Kerry S Bloom e Ed Salmon. 2010. "Meccanismi di generazione della forza mediante attacchi cinetocoro-microtubuli terminali". Opinione attuale in biologia cellulare 22 (1). Elsevier Ltd: 57-67. [<a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2822009/">Sorgente</a>]</li>
	<li>Khodjakov, Alexey e Tarun Kapoor. 2005. "Flusso di microtubuli: a cosa serve?" Biologia attuale 15 (23). Elsevier Inc: R966–R968. [<a href="https://www.sciencedirect.com/science/article/pii/S0960982205013928">Sorgente</a>]</li>
</ol>

anafase a e b

I microtubuli si formano attraverso la polimerizzazione end-to-end degli eterodimeri di tubulina. I microtubuli del cinetocore hanno origine dai poli del fuso e le loro estremità positive si collegano con i cinetocori sui cromatidi fratelli. I complessi proteici Ndc80, presenti sul cinetocore, formano collegamenti a bassa affinità con l'estremità positiva di questi microtubuli del cinetocore.

La depolimerizzazione dell'estremità positiva rilascia eterodimeri di tubulina dalla regione terminale del microtubulo. Quando le subunità della tubulina vengono perse, i complessi Ndc80 si staccano e si riattaccano nei siti davanti al segmento di depolimerizzazione del microtubulo. Il processo si traduce in uno spostamento verso i poli, attirando il cinetocore e il cromatide associato verso il polo del fuso.

Il flusso dei microtubuli attira i cromatidi verso i poli del fuso.

Le subunità di tubulina che formano il reticolo dei microtubuli si muovono continuamente verso l'estremità negativa, esibendo un flusso di microtubuli diretto all'estremità negativa.

Il flusso dei microtubuli si sviluppa quando la depolimerizzazione continua all'estremità negativa è bilanciata dalla polimerizzazione continua all'estremità positiva. Una velocità costante di depolimerizzazione e polimerizzazione mantiene una lunghezza fissa dei microtubuli mentre le singole subunità all'interno del reticolo si muovono verso l'estremità di depolimerizzazione.

I microtubuli del cinetocoro sottoposti a flusso trascinano i cinetocori e i cromatidi associati lungo la direzione del flusso, verso i poli del fuso.

Proteine motrici dei microtubuli – Dineina e Kinesina-5

Le dineine sono proteine motorie dirette all'estremità negativa dei microtubuli. Le dineine collegano le estremità positive dei microtubuli astrali con i componenti citoscheletrici nella corteccia cellulare, posizionando così i poli del fuso all'interno della cellula. Il movimento diretto delle dineine verso l'estremità negativa genera una forza che attira i poli del fuso verso la corteccia cellulare.

Le kinesine-5 sono proteine motorie dirette all'estremità positiva. La kinesina-5 interagisce con le estremità positive dei microtubuli interpolari antiparalleli nella zona centrale del fuso. Questi motori dei microtubuli aiutano i microtubuli interpolari a scorrere uno accanto all'altro generando una forza che allontana i poli del fuso.

Anafase A e B

I microtubuli si formano attraverso la polimerizzazione end-to-end degli eterodimeri di tubulina. I microtubuli cinetocore hanno origine dai poli del fuso e il loro ...

Education

Didattica

JoVE Core

Molecular Biology

Biologia Molecolare

Unit 2

Unità 2

Cell Division

Divisione cellulare

KEY TERMS AND CONCEPTS

TERMINI E CONCETTI CHIAVE

chromatid movement by microtubule forces

Chromatid movement during anaphase depends on microtubule forces at the spindle. Kinetochore microtubules grow from the spindle poles, and their plus ends attach to kinetochores on sister chromatids. The kinetochore protein complex Ndc80 makes low-affinity links with the microtubule plus end.

Chromatid Movement by Microtubule Forces

mitosis and cytokinesis

Mitosis and Cytokinesis Stages

Mitosis and cytokinesis are the two main steps of cell division. Mitosis is the process that divides the nucleus, while cytokinesis splits the rest of the cell. During mitosis, the cell’s genetic material is separated into two sets. This helps form two new nuclei with the same genetic information. Cytokinesis then completes division by separating the cytoplasm, which creates two daughter cells. These stages work together to make cell division possible in growing and repairing tissues.

duplication of chromatin structure

Chromatin Rebuilding During DNA Replication

Chromatin rebuilding during DNA replication helps cells copy DNA and pass on chromosome structure to daughter cells. During cell division, the genome must be disrupted and then reassembled across the whole chromosome. This process allows chromatin to be inherited, rebuilt, and maintained in new cells. The basic unit of chromatin is the nucleosome. A nucleosome contains DNA wrapped around an octamer of histone proteins, with short linker DNA between neighboring nucleosomes. The histone proteins...

cohesins

Cohesins in Meiosis and Genetic Disorders

Cohesin protein complexes act like molecular glue that keeps sister chromatids together. They are important in both mitosis and meiosis. In mitosis, cohesin complexes are removed from chromosomes before anaphase begins. Meiosis has two rounds of chromosome separation. Meiosis I separates homologous chromosomes, while Meiosis II separates sister chromatids. This process produces four daughter cells. The Meiosis I cohesin complex is made of four subunits: Smc1, Smc3, Rec8, and Scc3. Rec8...

condensins

How Condensins Shape Mitotic Chromosomes

Condensins shape chromosomes during mitosis, the stage when a cell divides. These large protein complexes use ATP, the cell’s energy molecule, to help turn loose post-interphase DNA into compact, separate chromosomes. They organize and segregate chromosomal DNA so it can be passed on correctly. Plant and animal cells contain two condensin complexes: condensin I and condensin II. Each complex has five subunits. Two are SMC, or Structural Maintenance of Chromosomes, subunits. One is a kleisin...

the mitotic spindle

How the Mitotic Spindle Separates Chromosomes

The mitotic spindle is a microtubule-based structure that helps separate chromosomes during cell division. It forms in eukaryotic cells and moves sister chromatids to opposite ends of the parent cell. This leaves each new cell nucleus with the correct genetic material. A bipolar spindle has two poles, and that shape is important for chromosome segregation. In many animal cells, two centrosomes are present when mitosis begins. Each centrosome helps organize a circular set of microtubules at one...

centrosome duplication

Centrosome Duplication in the Cell Cycle

Centrosome duplication is a key part of the animal cell cycle. The centrosome is the main microtubule organizing center, or MTOC, in animal cells. It contains two cylindrical centrioles at its core. Each centriole has nine sets of three microtubules held together by proteins. The two centrioles sit at right angles and are surrounded by the pericentriolar matrix, or PCM, a shapeless protein cloud. Duplication starts early in the cell cycle so each daughter cell can receive a centrosome after...

microtubule instability

Microtubule Growth and Shrinkage

Microtubules are hollow, cylindrical filaments in the cell. They are about 25 nm wide and can range from 200 nm to 25 μm in length. Their main building blocks are GTP-bound tubulin subunits that form αβ-heterodimers, which then assemble into protofilaments. These protofilaments join by lateral bonds to create stable microtubule cylinders. Microtubules are highly dynamic because they repeatedly assemble and break down. They can even grow on one end while shrinking on the other at the same time.

spindle assembly

How the Mitotic Spindle Forms

The mitotic spindle forms through centrosome-mediated, chromatin-mediated, and microtubule-mediated pathways. These pathways often work at the same time. Together, they build a strong spindle apparatus for cell division. In most cells, centrosomes are the main sites where microtubules begin to grow. During the G2 to prophase transition, centrosomes mature and make more microtubules. The growing microtubules extend from both centrosomes, and their plus-ends search for chromosomes by attaching...

attachment of sister chromatids

Kinetochore-Microtubule Binding in Mitosis

Kinetochore-microtubule binding helps chromosomes line up and separate during mitosis. After the nuclear envelope breaks down, condensed chromosomes are exposed to the bipolar microtubules of the mitotic spindle. The kinetochore is a large, disc-shaped protein complex at the centromere region of each sister chromatid, and it serves as the microtubule binding site. Usually, the plus-end of a single microtubule inserts into the kinetochore. In some cases, a kinetochore first makes lateral...

forces acting on chromosomes

Chromosome Movement During Mitosis

Chromosome movement during mitosis depends on several small forces that act at different times and positions in the cell. In prometaphase, these forces help chromosomes move to the equatorial plane and line up at the metaphase plate. Because the forces change with location and stage, chromosome behavior also changes as mitosis continues. Microtubules and motor proteins create two main kinds of force on chromosomes: poleward force and anti-poleward force, also called polar-ejection force.

separation of sister chromatids

Anaphase Start and Chromatid Release

Anaphase starts when sister chromatids are released from one another and pulled toward opposite spindle poles. Before that happens, cohesion along the chromosomal arms is reduced from prophase to metaphase, which helps the sister chromatids resolve. A residual cohesin link still holds them together until the metaphase-to-anaphase transition. This leftover connection helps prevent early separation and reduces the risk of aneuploidy, or the wrong number of chromosomes, in daughter cells. At the...

the spindle assembly checkpoint

How Cells Safeguard Chromosome Separation

The spindle assembly checkpoint helps cells safeguard chromosome separation during anaphase. It acts as a molecular surveillance system. The checkpoint checks whether the steps needed for chromosome segregation are complete before the cell moves on. Many proteins work together to control this checkpoint. If mutations affect these proteins, a cell may enter anaphase too early. Chromosomes can then separate before the needed steps are finished. When this happens, the daughter cells may receive...

anaphase a and b

Chromatid movement during anaphase depends on microtubule forces at the spindle. Kinetochore microtubules grow from the spindle poles, and their plus ends attach to kinetochores on sister chromatids. The kinetochore protein complex Ndc80 makes low-affinity links with the microtubule plus end.

the contractile ring

How Cells Build the Contractile Ring

Contractile rings are built from microfilaments and help separate daughter cells during cytokinesis. This ring forms as the cell cycle moves forward, but the exact timing and coordination with other cell cycle events are still not fully understood. RhoA, a small GTPase in the Ras superfamily, controls contractile ring assembly and function. The inactive form of RhoA is bound to GDP. A guanine nucleotide exchange factor, or Rho-GEF, is located in the cortex region, where the next cell division...

determining the plane of cell division

How Cells Set the Division Plane

How cells set the division plane during cell division affects daughter cell size and, in many cases, cell fate. The plane of division is perpendicular to the plane of chromosome segregation. Different organisms use different cues to place it, based on their shape and needs. In animal cells, the cleavage furrow forms along the division plane from the cell cortex, the area just below the plasma membrane. The mitotic spindle is the main guide for this position. If the spindle axis changes, the...

the phragmoplast

How Plant Cells Build a New Wall

Plant cells build a new wall during cytokinesis with help from the phragmoplast. This plant structure forms from the central spindle and related cell parts as the cell finishes dividing. In animal cells, the comparable structure is the midbody, but in plants the phragmoplast expands outward to guide new wall formation. The mature phragmoplast has a donut-like shape. It has an outer leading region, a middle transition zone, and an inner lagging region. New microtubules are assembled...

distribution of cytoplasmic content

How Organelles Are Split During Cytokinesis

Cytokinesis splits a cell’s chromosomes and organelles into two daughter cells. Before cell division, organelles grow and divide. They cannot be made from scratch, so each daughter cell must inherit at least one copy to survive. Many details of this organelle sorting process are still not fully known. The cytoplasm contains organelles, salts, proteins, and water. Small organelles such as peroxisomes and lysosomes can be shared by simple diffusion. Because cells contain many copies of these...

anaphase promoting complex

APC Activates Chromosome Separation

The anaphase-promoting complex, or APC, helps control cell cycle progression by destroying specific proteins at the right time. These proteins are tagged with ubiquitin by ubiquitin ligases and then broken down by the proteasome. The SCF (Skp1/Cullin/F-box) complex and APC are two major ubiquitin ligases involved in this process. SCF stays active throughout the cell cycle, but APC turns on during the metaphase-to-anaphase transition. APC becomes active when Cdc20 or Cdh1 binds to it. These...

key terms and concepts

See how kinetochore microtubules, Ndc80, microtubule flux, dynein, and kinesin-5 help move chromatids and position the spindle during anaphase.

Chromatid Movement by Microtubule Forces | JoVE K12

molecular biology

Le video animazioni di JoVE Core Molecular Biology, organizzate in video lezioni, illustrano i processi molecolari cellulari in maniera chiara e concisa. Inoltre, nei video di "scienziati in azione", i concetti scientifici vengono applicati e dimostrati da ricercatori attraverso esperimenti in laboratorio.

new-york-state-science-learning-standards

New York State Science Learning Standards

Inheritance and Variation of Traits

HS-LS1-4.

Characteristics of Life

Biology is a natural science that studies life and living organisms, including their structure, function, development, interactions, evolution, ...

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Cytoplasm

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The Central Dogma

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Nucleic Acids

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Chromosome Structure

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Globular and Fibrous Proteins

Translation

Translation is the process of synthesizing proteins from the genetic information carried by messenger RNA (mRNA). Following transcription, it constitutes ...

Intrinsically Disordered Proteins

Ribosomes

Ribosomes translate genetic information encoded by messenger RNA (mRNA) into proteins. Both prokaryotic and eukaryotic cells have ribosomes. Cells that ...

Protein Complex Assembly

Protein and Protein Structures

Conjugated Proteins

Proteins: From Genes to Degradation

Amyloid Fibrils

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Proteins are polymers of amino acids linked together by peptide bonds. Proteins and polypeptides are interchangeably used to refer to long chains of amino ...

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Proteins are polymers of amino acid residues. They are versatile and responsible for different cellular functions, including DNA replication, molecular ...

Protein-Protein Interfaces

Protein Folding

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Cofactors and Coenzymes

Enzymes are proteins made of amino acids. The functional group of each constituent amino acid catalyzes a wide variety of chemical reactions via ionic ...

Cooperative Allosteric Transitions

Protein Kinases and Phosphatases

GTPases and their Regulation

Covalently Linked Protein Regulators

protein-complexes-with-interchangeable-parts

Protein Complexes with Interchangeable Parts

Mechanical Protein Function

Structural Protein Function

Protein Networks

Construct an explanation based on evidence for how the structure of DNA determines the structure of proteins, which carry out the essential functions of life through systems of specialized cells. [Assessment Boundary: Assessment does not include identification of specific cell or tissue types, whole body systems, specific protein structures and functions, or the biochemistry of protein synthesis.]

HS-LS1-1

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structural-organization-of-the-human-body-an-overview

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Anatomy of the Intestines

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Accessory Organs

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Lipid Digestion

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Protein Digestion

Protein digestion begins in the stomach, where the highly acidic environment can easily disrupt protein structure by exposing the peptide bonds of ...

Carbohydrate Digestion

Carbohydrate digestion and metabolism break down simple and complex carbohydrates from food into saccharides (i.e., sugars) for the body to use as energy. ...

What is a Nervous System?

Overview

The nervous system is the collection of specialized cells responsible for maintaining an organism&rsquo;s internal environment and coordinating ...

The Parasympathetic Nervous System

Overview

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The Sympathetic Nervous System

Overview

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Oogenesis

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Sensory systems detect stimuli&mdash;such as light and sound waves&mdash;and transduce them into neural signals that can be interpreted by the nervous ...

Fertilization

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an-introduction-to-the-zebrafish-danio-rerio

An Introduction to the Zebrafish: Danio rerio

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Physiology of the Circulatory System

Homeostasis
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Anatomy of the Heart

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ExpandBefore starting the experiment go to the front of the room and collect an alcohol swab, a ...

Blood Flow

Blood is pumped by the heart into the aorta, the largest artery in the body, and then into increasingly smaller arteries, arterioles, and capillaries. The ...

Develop and use a model to illustrate the hierarchical organization of interacting systems that provide specific functions within multicellular organisms. [Clarification Statement: Emphasis is on functions at the organism system level such as nutrient uptake, water delivery, and organism movement in response to neural stimuli. An example of an interacting system could be an artery depending on the proper function of elastic tissue and smooth muscle to regulate and deliver the proper amount of blood within the circulatory system.] [Assessment Boundary: Assessment does not include interactions and functions at the molecular or chemical reaction level.]

HS-LS1-2

Chromatid Movement by Microtubule Forces

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