Mouse Tissue
Mouse Tissue - Related Videos
02:46
Immunostaining of Mouse Craniofacial Tissues
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2025
This video demonstrates the immunofluorescence staining of cryosections from mouse embryonic craniofacial tissues, using fluorescence microscopy to assess nuclear marker expression and determine cell proliferation and signaling activity.
12:39
Rapid Genotyping of Mouse Tissue Using Sigma's Extract-N-Amp Tissue PCR Kit
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Cited by 4 •
2008
The complete genotyping of a mouse tail sample, including tissue digestion and PCR readout, is done in one and a half hours using Sigma's SYBR Green Extract-N-Amp Tissue PCR kit.
02:37
Frozen Mouse Brain Tissue Sectioning: A Procedure to Obtain Thin Frozen Tissue Sections from Frozen Murine Brain Tissue
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2023
In this video, we demonstrate the sectioning of a frozen mouse brain tumor tissue using a cryostat. The frozen brain tissue sections so obtained are stored at low temperatures until further analysis.
08:47
Preparation of Mouse Brain Tissue for Immunoelectron Microscopy
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Cited by 52 •
2010
We describe a protocol for transcardiac perfusion of mice, removal and sectioning of the brain, as well as immunoperoxidase staining, resin embedding, and ultrathin sectioning of the brain sections. Upon completion of these procedures, the immunostained material is ready for examination with transmission electron microscopy.
06:59
Isolation of Primary Myofibroblasts from Mouse and Human Colon Tissue
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Cited by 43 •
2013
The myofibroblast is an influential stromal cell of the gastrointestinal tract that regulates important physiologic processes in both normal and disease states. We describe a technique that allows for the isolation of primary myofibroblasts from both mouse and human colon tissue, which can be utilized for in vitro experimentation.
FAQs
The main biochemical purpose of homogenization is to disrupt the tissue in controlled conditions so its molecular constituents become accessible for downstream separation and measurement. Researchers then select extraction conditions according to whether they are examining proteins, nucleic acids, metabolites, or membrane components. Maintaining controlled handling helps make differences between samples more interpretable.
Centrifugation provides a separation stage after homogenization, helping researchers organize the resulting material before analysis. Used together with extraction methods, it supports the preparation of fractions enriched for proteins, nucleic acids, metabolites, or membrane components. This separation allows biochemical measurements to focus on selected molecular contents rather than treating the homogenized sample as a single undifferentiated mixture.
Comparing samples can reveal changes in enzyme activity, signaling pathways, and metabolism, as well as responses associated with disease or treatment. The value of the comparison depends on examining samples under appropriately controlled conditions, so observed molecular differences can be related to the biological condition being studied. These measurements connect biochemical variation with physiological function.
Measurements from mouse tissue help place molecular observations within a whole-animal biological context. Instead of examining an isolated biochemical component alone, researchers can relate protein, nucleic acid, metabolite, or membrane findings to tissue function and physiological changes. This connection supports interpretation of molecular mechanisms in studies involving pharmacology, genetics, immunology, and disease models.
A typical workflow begins by homogenizing the tissue under controlled conditions. Researchers then use centrifugation and extraction methods to separate the molecular material relevant to the experiment, such as proteins, nucleic acids, metabolites, or membrane components. The resulting preparations can be analyzed and compared across samples to investigate enzyme activity, signaling, metabolism, or treatment responses.
Researchers use mouse tissue when they need biochemical measurements linked to physiological or disease-related changes. Applications include examining pharmacological effects, studying genetic mechanisms, investigating immune processes, and modeling disease. Analyses may identify altered signaling, enzyme activity, or metabolism, producing findings that clarify biological mechanisms and may inform studies relevant to human health.