Bodipy 493/503
Bodipy 493/503 - Related Videos
10:58
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Cited by 19 •
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In honey bee workers, aging depends on social behaviors rather than on chronological age. Here we show how worker-types with very different aging patterns can be obtained and analyzed for cellular senescence.
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Cited by 8 •
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Here we describe an adaptation of protocols used to induce homeostatic plasticity in neurons for the study of plasticity of astrocytic G protein-coupled receptors. Recently used to examine changes in astrocytic group I mGluRs in juvenile mice, the method can be applied to measure scaling of various astrocytic GPCRs, in tissue from adult mice in situ and in vivo, and to gain a better appreciation of the sensitivity of astrocytic receptors to changes in neuronal activity.
14:23
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Proteome analysis of the cochlear sensory epithelium can be challenging due to its small size and because membrane proteins are difficult to isolate and identify. Both membrane and soluble proteins can be identified by combining multiple preparative methods and separation techniques along with high-resolution mass spectrometry.
FAQs
The dye’s hydrophobic structure drives it toward nonpolar lipid environments rather than aqueous cellular regions. Within neutral lipid droplets, BODIPY 493/503 becomes concentrated, producing a stronger fluorescent signal than when it is dispersed. This partitioning is the key chemical basis for using the probe to visualize lipid storage and examine how neutral-lipid accumulation changes under different biochemical conditions.
Excitation near 493 nm and emission near 503 nm provide the optical basis for collecting the signal. A microscope illuminates the labeled sample at the excitation range and records emitted fluorescence near the emission range. Keeping these wavelength roles distinct helps researchers identify the dye’s signal and compare lipid-droplet fluorescence across cells, tissues, or experimental conditions.
Signal intensity is linked to the dye’s concentration in nonpolar lipid environments, especially neutral lipid droplets. Consequently, stronger localized fluorescence can reveal where these storage structures occur, while repeated imaging can help follow their distribution over time. The readout is therefore useful for connecting droplet-level localization with broader questions about lipid homeostasis and metabolism.
An experiment generally labels cultured cells or tissue with BODIPY 493/503 and examines the sample by fluorescence microscopy. Researchers then detect fluorescent lipid droplets, measure the resulting droplet signal, and track changes when samples are compared across conditions. This workflow connects an optical image to cellular lipid-storage behavior without requiring the overview-level interpretation to be repeated.
Fluorescence microscopy with BODIPY 493/503 can support detection, measurement, and tracking of droplets in cultured cells and tissues. Those observations allow investigators to evaluate lipid storage and changes in lipid homeostasis, including responses associated with nutrient or drug treatment, while retaining spatial information about where the signal occurs.
Neutral lipid droplets provide a visible readout for studying cellular energy storage and lipid handling. Applying the dye in cell or tissue studies can therefore support investigations of lipid metabolism, metabolic disease, and cellular responses to altered nutrient or drug conditions. Its microscopy-based readout links biochemical questions to observable changes in storage structures.