Constitutive Cre Expression
Constitutive Cre Expression - Related Videos
01:27
Constitutive and Regulated Gene Expression
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2025
Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
10:03
Development of Leishmania Species Strains with Constitutive Expression of eGFP
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2023
Here, we describe the methodology used for generating L. panamensis and L. donovani strains expressing the gene for eGFP as a stable integrated transgene using the pLEXSY system. Transfected parasites were cloned by limiting dilution, and clones with the highest fluorescence intensity in both species were selected for further use in drug screening assays.
02:45
Assessment of Viral Fusion Inhibitors Using a Cre-Inducible Fluorescent Reporter
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2026
Source: Esposito, A. M.,et al. High-throughput Cre-Lox Activated Viral Membrane Fusion Assay to Identify Inhibitors of HIV-1 Viral Membrane Fusion. J. Vis. Exp. (2018)This video demonstrates a co-culture fluorescent assay to monitor cell-to-cell viral transmission. The assay uses a red-to-green reporter cassette to visualize viral membrane fusion and evaluate the efficacy of test compounds in blocking viral entry.
08:22
Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference
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Cited by 2 •
2013
This article describes how to microinject viral vectors into mouse brain and then test in a conditioned place preference paradigm that includes an acquisition, extinction and reinstatement phase.
08:19
Quantitative Comparison of cis-Regulatory Element (CRE) Activities in Transgenic Drosophila melanogaster
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Cited by 13 •
2011
Phenotypic variation for traits can result from mutations in cis-regulatory element (CRE) sequences that control gene expression patterns. Methods derived for use in Drosophila melanogaster can quantitatively compare the levels of spatial and temporal patterns of gene expression mediated by modified or naturally occurring CRE variants.
FAQs
The outcome is determined by the loxP configuration around the DNA segment. When the sites flank a region in a compatible arrangement, Cre can remove that segment; alternative orientations can produce inversion or activation. Thus, the same recombinase system can be engineered for different genetic readouts, including loss of gene function or expression of a reporter.
Continuous production gives Cre repeated opportunities to act wherever the recombinase and loxP-targeted DNA are present. This supports genetic changes throughout development or across a defined tissue population rather than restricting recombination to a single moment. The resulting modification can then remain relevant for evaluating gene function or cell behavior over extended biological periods.
Recombination can create a lasting DNA state even after Cre is no longer being produced. Consequently, a cell may retain a deletion, inversion, or activated genetic configuration as its descendants develop. This persistence is important when experiments examine long-term gene effects, stable reporter behavior, or the fate of cells that experienced the original recombination event.
Researchers can match the loxP arrangement to the intended genetic outcome. A design that removes a selected DNA segment can support conditional gene knockout, whereas another arrangement can generate inversion or activation. This choice links the physical organization of loxP sites to the experiment’s readout, allowing one Cre-based framework to support different engineered genetic functions.
Constitutive Cre systems support several complementary applications: conditional knockout models for testing gene function, reporter models for marking recombined cells, and lineage tracing for following cell fate. Because the resulting DNA rearrangement can be durable, these applications can connect an early genetically defined event with later observations of development, tissue behavior, or long-term biological outcomes.
In engineered cell-line construction, a genetically defined population can be designed to undergo a stable Cre-mediated DNA change. Depending on the loxP arrangement, the resulting line may carry a rearranged, deleted, inverted, or activated genetic segment. Such stable configurations provide a foundation for studying gene function or maintaining reporter-based genetic states in subsequent analyses.