Human Venous Saphenous Cells
Human Venous Saphenous Cells - Related Videos
11:35
Decellularization and Recellularization Methodology for Human Saphenous Veins
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Cited by 21 •
2018
Here, we describe a protocol for the saphenous vein decellularization using detergents and recellularization by perfusion of the peripheral blood and the endothelial medium.
06:02
Anatomical Reconstructions of the Human Cardiac Venous System using Contrast-computed Tomography of Perfusion-fixed Specimens
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Cited by 9 •
2013
The objective of this research is to recreate and then access the anatomy of the human cardiac venous system using 3D reconstructions generated from contrast-computed tomography scans.
09:50
Procedure for Human Saphenous Veins Ex Vivo Perfusion and External Reinforcement
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Cited by 9 •
2014
The mechanisms leading to the development of intimal hyperplasia (IH) and vein graft failure are still poorly understood. This study describes an ex vivo system to perfuse human veins under controlled flow and pressure. Furthermore the efficiency of external mesh reinforcement to limit the development of IH was evaluated.
05:27
Drawing Blood from Rats through the Saphenous Vein and by Cardiac Puncture
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Cited by 87 •
2007
Blood draws are necessary in a large number of studies, for example to study the pharmacokinetics profile of a compound. Here, we demonstrate how to draw blood from rats using two techniques: blood draw from the saphenous vein or by cardiac puncture.
09:10
Human Saphenous Vein Endothelial Cell Isolation and Exposure to Controlled Levels of Shear Stress and Stretch
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Cited by 1 •
2023
We describe a protocol to isolate and culture human saphenous vein endothelial cells (hSVECs). We also provide detailed methods to produce shear stress and stretch to study mechanical stress in hSVECs.
FAQs
Adhesion establishes contact between the cells and a candidate biomaterial surface, allowing researchers to examine how that interface supports cellular behavior. Because attached cells can respond to the surrounding material, adhesion provides an early indication of vascular compatibility. These observations help guide the selection and design of surfaces intended for tissue-engineered vessels or vascular grafts.
Biochemical and mechanical cues can alter how the cells behave in culture, including their proliferation and extracellular-matrix activity. Studying these responses helps researchers determine whether a biomaterial or engineered environment promotes desirable vascular tissue behavior. The combined cues are important because implantation conditions are not defined by material chemistry alone, but also by physical signals.
Extracellular matrix provides structural material that contributes to vessel organization and function. Human venous saphenous cells can produce or remodel this matrix, so their activity offers insight into how engineered tissues may develop and change over time. Measuring this behavior helps researchers assess whether a construct is likely to support appropriate tissue structure rather than merely cell attachment.
Human origin provides clinically relevant information about how vascular cells may interact with materials and implantation-related conditions. This relevance supports evaluation of vascular compatibility and tissue remodeling in a human biological context. Consequently, findings from these cultures can inform regenerative strategies for repairing or replacing damaged veins, while connecting laboratory observations to intended clinical applications.
A study can begin with primary cells obtained from the saphenous vein, followed by culture on a selected biomaterial surface. Researchers then examine cellular responses to the culture environment, including adhesion, proliferation, and extracellular-matrix production or remodeling. This workflow links material contact and environmental cues to outcomes relevant to vascular tissue design.
They are useful when a study needs to determine how a proposed graft material interacts with human vascular cells. Researchers can observe whether the surface supports adhesion and proliferation and whether cellular matrix activity changes in response to it. These outcomes provide evidence about vascular compatibility and help identify materials more suitable for engineered blood-vessel applications.
Their responses to biomaterial surfaces and biochemical or mechanical cues help researchers study the cellular processes that influence engineered vessel structure and function. In particular, proliferation and extracellular-matrix production or remodeling reveal how cells may contribute to tissue development. This information can guide construction of vascular tissues designed for repair or replacement purposes.
Cultured cells provide a way to investigate how implantation-related biochemical and mechanical conditions may affect vascular cellular behavior. Observing changes in adhesion, proliferation, and matrix remodeling helps researchers evaluate compatibility and predict tissue responses. Such evidence contributes to the design of regenerative approaches aimed at repairing damaged veins or replacing vascular tissue with engineered constructs.