Ion Concentration Measurement
Ion Concentration Measurement - Related Videos
08:17
Fluorescent Nanoparticles for the Measurement of Ion Concentration in Biological Systems
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Cited by 24 •
2011
Fluorescent nanoparticles produced in our lab are used for imaging ion concentrations and ion fluxes in biological systems such as cells during signaling and interstitial fluid during physiological homeostasis.
03:41
Measurement of Evoked Potassium Ion Concentration Dynamics in Coronal Hippocampal Slices
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2025
The video demonstrates evoked potassium ion concentration dynamics on electrical stimulation of mouse coronal hippocampal brain slices. A bipolar stimulating electrode is inserted into the stratum radiatum of the CA3 region in the hippocampus, and a potassium-ion selective electrode is placed in the CA1 stratum radiatum. The electrical stimulation causes an action potential, resulting in potassium ion release. Finally, the lack of evoked potassium ion response in the presence of an inhibitory...
11:08
Double-barreled and Concentric Microelectrodes for Measurement of Extracellular Ion Signals in Brain Tissue
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Cited by 19 •
2015
We demonstrate the fabrication, calibration and properties of two types of ion-selective microelectrodes (double-barreled and concentric) for measurement of ion concentrations in brain tissue. These are then used in the mouse hippocampal slice preparation to show that excitatory activity changes both extracellular potassium and sodium concentrations.
08:06
Merging Ion Concentration Polarization between Juxtaposed Ion Exchange Membranes to Block the Propagation of the Polarization Zone
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2017
The protocol for a novel ion concentration polarization (ICP) platform that can stop the propagation of the ICP zone, regardless of the operating conditions is described. This unique ability of the platform lies in the use of merging ion depletion and enrichment, which are two polarities of the ICP phenomenon.
09:18
Measurement of Extracellular Ion Fluxes Using the Ion-selective Self-referencing Microelectrode Technique
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Cited by 6 •
2015
Transporters in cell membranes allow differential segregation of ions across cell membranes or cell layers and play crucial roles during tissue physiology, repair and pathology. We describe the ion-selective self-referencing microelectrode that allows the measurement of specific ion fluxes at single cells and tissues in vivo.
FAQs
The electrical potential generated by an ion-selective electrode responds directly to ion activity, which reflects the ion’s effective chemical behavior in solution. Calibration provides the relationship needed to interpret that potential in terms of concentration. This distinction matters in biological and engineered environments, where ionic conditions can affect how accurately measured values represent the solution itself.
The membrane provides the electrode’s selectivity by preferentially interacting with a particular target ion. That interaction produces a measurable electrical potential while reducing the contribution of other dissolved species. Because the membrane determines which ion the sensor primarily responds to, it is central to obtaining measurements that are useful for monitoring specific electrolytes or pH.
The Nernst equation relates an electrode’s electrical potential to the activity of the target ion. In practice, researchers first calibrate the electrode and then use the measured potential to estimate the corresponding ion concentration. This relationship converts an electrical signal into quantitative information suitable for comparing ionic conditions across biological systems or engineered materials.
A typical workflow begins by calibrating the ion-selective electrode so its potential can be related to the target ion’s concentration. The electrode is then used to measure the sample’s electrical response, and the calibrated relationship supports concentration estimation. This sequence is important when monitoring pH, electrolytes, or other ionic conditions in bioengineering environments.
Ion concentration measurements can track pH, electrolyte levels, and broader ionic conditions in cell culture, bioprocessing, biomaterials, and physiological fluids. Monitoring these variables helps characterize whether an environment provides suitable chemical conditions for biological activity or engineered performance. The resulting data can support evaluation and control of systems designed to maintain cellular function.
In tissue engineering, measurements help characterize ionic environments that influence cellular function and material behavior. In drug delivery and diagnostic devices, they support assessment of ionic conditions relevant to device or formulation performance. These uses extend the technique beyond isolated solution analysis by connecting quantitative ion data with the design and evaluation of bioengineered systems.