On-chip Fractionation
On-chip Fractionation - Related Videos
10:09
Fractional Distillation
0 Views •
2023
Source: Laboratory of Dr. Nicholas Leadbeater — University of Connecticut Distillation is perhaps the most common laboratory technique employed by chemists for the purification of organic liquids. Compounds in a mixture with different boiling points separate into individual components when the mixture is carefully distilled. The two main types of distillation are "simple distillation" and "fractional distillation", and both are widely used in organic chemistry laboratories. Simple distillation...
07:50
Chip-Based Digital PCR to Detect Rare Transcript Variants Using a Nanofluidic Chip
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2025
This video demonstrates chip-based digital PCR — a variation of the digital PCR technique that is useful in detecting rare transcript variants. The PCR reaction is partitioned into the chambers of a nanofluidic chip, each of which acts as an independent reaction. The detection of fluorescence signals from the chambers with amplified targets confirms the presence of rare transcript variants in the sample.
01:35
Organ-on-Chip Models
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2026
Introduction to Organ-on-Chip Models Organ-on-chip models are advanced microphysiological systems that recreate key structural and functional features of human organs in a controlled laboratory setting. These devices integrate living human cells within microfluidic channels, allowing researchers to simulate tissue–tissue interfaces, fluid flow, and mechanical forces that are essential for normal organ function. Unlike traditional cell cultures, which often lack these dynamic physiological cues,...
11:23
Lensless Fluorescent Microscopy on a Chip
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Cited by 8 •
2011
A lensless on-chip fluorescent microscopy platform is demonstrated that can image fluorescent objects over an ultra-wide field-of-view of e.g., >0.6-8 cm2 with <4μm resolution using a compressive sampling based decoding algorithm. Such a compact and wide-field fluorescent on-chip imaging modality could be valuable for high-throughput cytometry, rare-cell research and microarray-analysis.
12:43
On-Chip Endothelial Inflammatory Phenotyping
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Cited by 3 •
2012
Microfluidic flow chambers etched by photolithography and fabricated from PDMS are applied to probe functional outcomes associated with EC dysfunction and inflammation. In a representative experiment, the ability of differential shear stress to modulate monocytic cell adhesion to cytokine activated EC monolayers is demonstrated.
FAQs
The separation mechanism depends on which physical property distinguishes the sample components. Differences in size, deformability, density, electrical properties, or hydrodynamic behavior can cause cells, particles, or biomolecules to follow different paths or become selectively retained. Designing the channel or barrier around the relevant property helps direct the desired fraction toward later analysis.
On-chip Fractionation can discriminate among components using several properties rather than relying on a single universal mechanism. Size and deformability are useful for distinguishing cells or particles, while density, electrical properties, and hydrodynamic behavior provide other separation bases. Selecting the appropriate property connects the device design to the composition and analytical goal of the sample.
Integration links separation with detection and other laboratory steps inside a miniaturized platform. This arrangement can reduce transfers between instruments, lower reagent use, and shorten processing times. It also supports portable and automated systems, making the workflow more suitable when researchers need efficient analysis from small sample volumes rather than a sequence of separate laboratory operations.
A typical workflow introduces a sample into a microfluidic device, directs the fluid through engineered channels or barriers, and allows components to separate according to their physical or hydrodynamic behavior. Distinct paths or selective retention produce separated fractions, which can then undergo detection or additional laboratory processing within the same integrated platform.
In bioengineering, On-chip Fractionation supports several targeted workflows, including cell sorting, sample preparation, circulating tumor cell enrichment, extracellular vesicle isolation, and biomolecule analysis. These applications use the device to separate relevant sample components before or alongside downstream analysis. The approach is especially useful when conserving sample and reagents is important.
Miniaturized fractionation systems can work with small sample volumes while combining separation with detection and other laboratory operations. This integration may reduce reagent consumption and processing time, and it can contribute to portable, automated platforms. Such features broaden the usefulness of fractionation for research and clinical applications that require compact and efficient workflows.