Temporal Bone Isolation
Temporal Bone Isolation - Related Videos
07:17
Murine Hind Limb Long Bone Dissection and Bone Marrow Isolation
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Cited by 293 •
2016
Here we present a protocol for the dissection of hind limb long bones (femurs and tibiae) from the laboratory mouse. We further describe a rapid technique for bone marrow isolation from these bones that utilizes centrifugation for removal of bone marrow from the bone marrow space.
11:52
Temporal Ordering of Dynamic Expression Data from Detailed Spatial Expression Maps
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2017
The segmentation clock drives oscillatory gene expression across the pre-somitic mesoderm (PSM). Dynamic Notch activity is key to this process. We use imaging and computational analyses to extract temporal dynamics from spatial expression data to demonstrate that Delta ligand and Notch receptor expression oscillate in the vertebrate PSM.
06:42
Extracting the Cochlea from a Human Temporal Bone: A Cadaveric Protocol
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2023
This article presents a reliable method for extracting the human cochlea from the cadaver temporal bone by drill-out while following distinct anatomical landmarks.
09:21
Isolation and Intravenous Injection of Murine Bone Marrow Derived Monocytes
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Cited by 31 •
2014
Here we present a protocol that generates large amounts of murine monocytes from heterogeneous bone marrow for translational applications. In comparison to others, this new method helps reduce the number of sacrificed animals and lowers costs by avoiding expensive methods such as high gradient magnetic cell separation (MACS).
09:53
Isolating Mesangiogenic Progenitor Cells (MPCs) from Human Bone Marrow
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Cited by 17 •
2016
Here we describe an optimized, highly reproducible protocol to isolate Mesodermal Progenitor Cells (MPCs) from human bone marrow (hBM). MPCs were characterized by flow cytometry and nestin expression. They showed the ability to give rise to exponentially growing MSC-like cell cultures while retaining their angiogenic potential.
FAQs
These structures provide the anatomical detail needed for meaningful engineering analysis. Their preservation maintains the relationships among the ear, surrounding bone, and facial nerve pathways rather than producing a simplified bony fragment. As a result, the specimen can support focused study of skull and ear biomechanics, device concepts, and clinically relevant experimental testing.
Defined cranial boundaries guide the cuts and establish which surrounding tissues are removed while keeping the target anatomy intact. Careful boundary selection helps produce a preparation that is sufficiently separated for focused analysis without sacrificing important ear or nerve-related structures. This balance directly affects the specimen’s usefulness for anatomical study and bioengineering evaluation.
An isolated specimen concentrates the anatomy associated with the skull and ear in a physical model that can be examined independently from surrounding cranial tissues. This supports investigation of structural relationships and mechanical behavior relevant to ear and skull systems. Such models also provide a basis for connecting anatomical observations with engineering analysis and device development.
Preparation begins by exposing the bone, followed by removal of attached soft tissue. The specimen is then separated by carefully cutting along defined cranial boundaries, while the external auditory canal, middle ear, inner ear, and facial nerve pathways are preserved. This sequence produces a focused preparation suitable for anatomical examination and subsequent engineering analysis.
The isolated preparation supplies a physical anatomical reference against which imaging-based reconstructions can be examined or validated. Because key ear and facial nerve-related structures remain preserved, the specimen can help connect reconstructed anatomy with the corresponding physical arrangement. This relationship is valuable when engineering studies require confidence that a model represents relevant temporal bone structures.
These preparations provide physical anatomical models for developing and evaluating hearing implants, while also supporting the design of surgical training systems. Preserved temporal bone structures allow engineering work to remain connected to clinically relevant anatomy. The same specimens can therefore contribute to device development, training-system design, and experimental testing that reflects the targeted cranial and ear region.