Bioreactor Cultivation
Bioreactor Cultivation - Related Videos
08:13
A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
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Cited by 8 •
2015
A novel reactor design, coined a high density bioreactor (HDBR), is presented for the cultivation and study of high density microbial communities. Here, the HDBR is successfully applied in a photobioreactor (PBR) configuration for the study of nitrogen metabolism by a mixed high density algal community.
11:42
Cultivation of Mammalian Cells Using a Single-use Pneumatic Bioreactor System
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Cited by 4 •
2014
Using a pneumatic bioreactor, we demonstrate the assembly, operation, and performance of this single-use bioreactor system for the growth of mammalian cells.
12:54
Operation of a Benchtop Bioreactor
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Cited by 9 •
2013
Fermentors are used to increase culture yield and productivity of bioengineered cells. After screening multiple microbial or animal cell culture candidates in shake flasks, the next logical step is to increase the selected culture’s biomass with the fermentor. This video demonstrates the setup and operation of a typical benchtop bioreactor system.
12:04
Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation
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Cited by 75 •
2013
In this protocol the fabrication, setup and basic operation of a microfluidic picoliter bioreactor (PLBR) for single-cell analysis of prokaryotic microorganisms is introduced. Industrially relevant microorganisms were analyzed as proof of principle allowing insights into growth rate, morphology, and phenotypic heterogeneity over certain time periods, hardly possible with conventional methods.
09:40
Batch and Continuous Bioreactors
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2023
Bioreactors are used to grow organisms in large volumes, thereby enabling the production of mass quantities of the target product. These reactors can be batch reactors, which contain all of the components needed for cell growth, or continuous reactors, which have inlet and outlet ports allowing for the addition of fresh growth media and the removal of cell waste. This video presents batch and continuous reactors and demonstrates the use of bioreactors to grow bacteria in the laboratory.
FAQs
Sensor-based control stabilizes cultivation by continuously tracking variables such as temperature, pH, dissolved oxygen, and nutrient availability. The control system uses these measurements to identify changes in the cellular environment and regulate operating conditions, helping maintain more uniform and reproducible growth. Continuous monitoring also creates a measurable basis for comparing runs and optimizing production performance.
Dissolved oxygen provides information about an environmental condition that supports cellular metabolism. Aeration supplies oxygen to the culture, while agitation helps distribute it through the vessel rather than allowing uneven regions to develop. Monitoring this variable allows the process to remain aligned with the requirements of the cultivated microorganisms, cells, or tissues and supports more consistent biological production.
Agitation and aeration work together to promote uniform conditions throughout the vessel. Mixing distributes nutrients, temperature, and dissolved oxygen, while aeration supports oxygen availability for cellular metabolism. Their coordinated use helps reduce environmental differences within the culture, which is important when researchers need reproducible growth and reliable production rather than conditions that vary from one region of the vessel to another.
A cultivation procedure can be organized around establishing and maintaining target conditions for temperature, pH, dissolved oxygen, nutrient availability, and mixing. Sensors measure these variables during the run, while agitation, aeration, and control systems help regulate the environment. Recording the measurements allows researchers to evaluate how conditions changed over time and identify opportunities for process optimization.
Bioreactor cultivation supports production of vaccines, enzymes, therapeutic proteins, biofuels, and cultured tissues. The controlled environment is useful when biological material must be grown reproducibly and evaluated under measured conditions. These applications connect laboratory biology with industrial production by providing a process that can be monitored, optimized, and used across different scales of research or manufacturing.
By controlling the cellular environment and measuring conditions continuously, bioreactor cultivation helps researchers compare results across runs and refine production conditions. Its use at laboratory and industrial scales supports a gradual transition from experimental findings toward larger-scale applications. In biology, this connection is especially relevant for processes intended to produce consistent vaccines, proteins, enzymes, fuels, or cultured tissues.