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JoVE Journal
Bioengineering

This content is Open Access.

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis
 

Microfluidic Picoliter Bioreactor for Microbial Single-cell Analysis: Fabrication, System Setup, and Operation

Article doi: 10.3791/50560
December 6th, 2013

Summary December 6th, 2013

In this protocol the fabrication, setup and basic operation of a microfluidic picoliter bioreactor (PLBR) for single-cell analysis of prokaryotic microorganisms is introduced. Industrially relevant microorganisms were analyzed as proof of principle allowing insights into growth rate, morphology, and phenotypic heterogeneity over certain time periods, hardly possible with conventional methods.

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