Dna Fingerprinting
Dna Fingerprinting - Related Videos
09:39
DNA Fingerprinting of Mycobacterium leprae Strains Using Variable Number Tandem Repeat (VNTR) - Fragment Length Analysis (FLA)
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Cited by 9 •
2011
Leprosy, caused by Mycobacterium leprae, is still endemic in many places. In order to learn about the spread and mode of transmission of leprosy, it is important to determine which strain of M. leprae has infected a patient. Variable numbers of tandem repeats (VNTR) typing is one such method.
08:35
Application of DNA Fingerprinting using the D1S80 Locus in Lab Classes
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Cited by 2 •
2021
Here we describe a simple protocol to generate DNA fingerprinting profiles by amplifying the VNTR locus D1S80 from epithelial cell DNA.
01:03
IR Frequency Region: Fingerprint Region
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2024
IR spectra are divided into two main regions: the diagnostic region and the fingerprint region. The diagnostic region of the spectrum lies above 1500 cm−1. The absorptions resulting from single-bond vibrations of the N–H, C–H, and O–H stretch at higher wavenumbers and appear on the left side of the spectrum. The stretching absorptions of the C≡C and C≡N occur between 2100–2300 cm−1. In contrast, those arising from stretching absorptions of the C=O, C=N, and C=C occur between 1600–1850 cm−1. The...
14:57
DNA Stable-Isotope Probing (DNA-SIP)
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Cited by 70 •
2010
DNA stable-isotope probing is a cultivation-independent method to identify and characterize active communities of microorganisms that are capable of utilizing specific substrates. Assimilation of substrate enriched in heavy isotope leads to incorporation of labelled atoms into microbial biomass. Density gradient ultracentrifugation retrieves labelled DNA for downstream molecular analyses.
12:02
Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
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Cited by 18 •
2016
We present an automated modular high-throughput-method for the identification and characterization of microbial exopolysaccharides in small scale. This method combines a fast preselection to analyze the total amount of secreted polysaccharides with a detailed carbohydrate fingerprint to enable the fast screening of newly isolated bacterial strains or entire strain collections.
FAQs
Short tandem repeats are useful because they vary substantially among individuals while remaining inherited. DNA fingerprinting targets these variable regions to create profiles that can distinguish biological samples. Because the differences are measurable and passed through biological inheritance, the resulting patterns support both individual identification and comparisons involving biological relationships.
PCR amplifies selected DNA regions, increasing the material available for analysis. The amplified fragments are then separated according to size, producing a pattern that can be examined as a genetic profile. These stages connect sequence variation at targeted regions with a visible comparison pattern, allowing profiles from different biological samples to be evaluated.
Relationship testing relies on the inherited nature of the DNA variations being compared. When profiles from biological samples are examined, shared patterns can provide evidence relevant to a biological relationship, while differing patterns help distinguish samples. The method therefore links molecular measurements to questions about inheritance rather than treating identification as an unrelated matching exercise.
A basic workflow starts with biological material, targets selected variable regions, amplifies those regions through PCR, and separates the resulting fragments by size. The observed fragment pattern is then compiled into a profile and compared with another profile. Each stage contributes a different function: targeting, amplification, size resolution, and comparison.
In forensic investigations, profile comparison can help distinguish samples associated with different individuals. The same approach can support identification of unknown remains, where an unknown biological sample is compared with available profiles. Its value comes from converting inherited genomic differences into evidence that can be evaluated across samples.
Within biology, DNA fingerprinting extends beyond individual identification by making genetic variation measurable across groups and research samples. Profile differences can contribute to studies of variation within populations and support work in conservation and medicine. This broader use shows how the same molecular evidence can address both organism-level identification and questions about inherited diversity.