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DNA Stable-Isotope Probing (DNA-SIP)
JoVE Journal
Biology
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JoVE Journal Biology
DNA Stable-Isotope Probing (DNA-SIP)

DNA Stable-Isotope Probing (DNA-SIP)

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14:57 min

August 02, 2010

DOI:

14:57 min
August 02, 2010

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Summary

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DNA stable-isotope probing is a cultivation-independent method to identify and characterize active communities of microorganisms that are capable of utilizing specific substrates. Assimilation of substrate enriched in heavy isotope leads to incorporation of labelled atoms into microbial biomass. Density gradient ultracentrifugation retrieves labelled DNA for downstream molecular analyses.

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