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DNA Stable-Isotope Probing (DNA-SIP)

DNA Stable-Isotope Probing (DNA-SIP)

Article DOI: 10.3791/2027
August 2nd, 2010


Summary August 2nd, 2010

DNA stable-isotope probing is a cultivation-independent method to identify and characterize active communities of microorganisms that are capable of utilizing specific substrates. Assimilation of substrate enriched in heavy isotope leads to incorporation of labelled atoms into microbial biomass. Density gradient ultracentrifugation retrieves labelled DNA for downstream molecular analyses.

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