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Journal / Biology / DNA Stable-Isotope Probing (DNA-SIP)
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DNA Stable-Isotope Probing (DNA-SIP)
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DNA Stable-Isotope Probing (DNA-SIP)

Article DOI: 10.3791/2027 • 14:58 min • August 2nd, 2010
August 2nd, 2010 •
1, 1
1Department of Biology, University of Waterloo

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Summary
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DNA stable-isotope probing is a cultivation-independent method to identify and characterize active communities of microorganisms that are capable of utilizing specific substrates. Assimilation of substrate enriched in heavy isotope leads to incorporation of labelled atoms into microbial biomass. Density gradient ultracentrifugation retrieves labelled DNA for downstream molecular analyses.

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DNA-SIP Stable-isotope Probing Microorganisms Carbon Substrates Nutrients Cellular Biomass Cultivation-independent Technique Metabolic Function Terrestrial Environments Aquatic Environments Stable-isotope Labelled Compounds Nucleic Acid Density Gradient Ultracentrifugation Gradient Fractionation DNA Purification Cesium Chloride Molecular Characterization Fingerprinting Microarrays Clone Libraries Metagenomics
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