Gingival Crevice Formation
Gingival Crevice Formation - Related Videos
09:47
Isolation, Processing and Analysis of Murine Gingival Cells
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Cited by 56 •
2013
This study describes an efficient technique to isolate and process gingival tissues from the mouse oral cavity in order to produce a single-cell culture. The resulting cells can be further used for flow cytometry analysis and molecular studies.
03:19
Isolation of Gingival Immune Cell Network from Murine Teeth Blocks
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2025
This video demonstrates the isolation procedure of the gingival immune cell network from a mouse model. The enzymatic digestion releases the gingival tissue from the attached teeth and bone, which is further mechanically digested, releasing immune cells from the gingiva. The isolated cells are ready for immunological experiments.
05:07
Isolation, Characterization and Functional Examination of the Gingival Immune Cell Network
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Cited by 30 •
2016
We have established a technique for the isolation, phenotypic characterization and functional analysis of immune cells from murine gingiva.
04:46
In Situ Detection of Bacteria in Mouse Gingival Tissue Sections
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2026
Source: Choi, Y. S., et al. In Situ Detection of Bacteria within Paraffin-embedded Tissues Using a Digoxin-labeled DNA Probe Targeting 16S rRNA. J. Vis. Exp. (2015).This video demonstrates the in situ detection of bacteria in mouse gingival tissue sections using digoxin-labeled DNA probes targeting bacterial DNA. The section is incubated with the probe. Alkaline phosphatase-conjugated antibodies specific to digoxin are added. Endogenous phosphatases are inactivated, and a colored precipitate is...
06:47
Isolation and Culture of Primary Human Gingival Epithelial Cells using Y-27632
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Cited by 4 •
2021
Here we present a modified method for the isolation and culture of human gingival epithelial cells by adding the Rock inhibitor, Y-27632, to the traditional method. This method is easier, less time-consuming, enhances stem cell properties, and produces larger numbers of high-potential epithelial cells both for the laboratory and for clinical applications.
FAQs
As the tooth erupts, the reduced enamel epithelium joins the oral epithelium and is reorganized into junctional epithelium. This reorganization establishes an attachment around the tooth while preserving a narrow sulcus at the tissue-tooth interface. The resulting dentogingival junction is therefore both an attachment structure and a controlled boundary where oral tissues meet the erupted tooth.
The remaining sulcus provides an interface rather than an uninterrupted epithelial seal. Its narrow space permits gingival crevicular fluid to enter, bringing immune components into a location exposed to microbial challenge. This arrangement links physical attachment with local monitoring, helping explain why changes at the crevice can affect barrier function and periodontal health.
Gingival crevicular fluid contributes immune components to the crevice, where they can help monitor microbial challenge. Its presence makes the space biologically active rather than merely anatomical. In studies of gingival crevice formation, examining this fluid helps connect development of the dentogingival junction with the oral barrier response and with conditions that may support periodontal health.
A study of this process would follow the relationship between tooth eruption and epithelial remodeling. Key observations would include union of the reduced enamel epithelium with oral epithelium, formation of junctional epithelium, attachment around the tooth, persistence of the sulcus, and entry of gingival crevicular fluid. Together, these features show how structure and immune surveillance develop at one interface.
When the developing or established dentogingival interface is disrupted, plaque may accumulate more readily near the tooth, and inflammation can follow. This connection makes crevice formation relevant to periodontal disease research because investigators can relate altered barrier organization to microbial challenge and to changes in periodontal health. The crevice therefore provides a site for examining how local structural disruption may have broader consequences.
The process provides a biological framework for studying how oral tissues balance attachment and exposure after eruption. It connects epithelial organization, a narrow sulcus, fluid-borne immune monitoring, and microbial challenge in one site. In biology and periodontal research, this framework helps interpret how a normally functioning dentogingival junction differs from one associated with plaque accumulation, inflammation, or disease.