Intestinal Kidney Cells
Intestinal Kidney Cells - Related Videos
08:19
The Mouse Isolated Perfused Kidney Technique
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Cited by 24 •
2016
The mouse isolated perfused kidney (MIPK) is a technique for keeping a mouse kidney under ex vivo conditions perfused and functional for 1 hr. The buffers and surgical technique are described in detail.
06:56
Isolation of Double Negative αβ T Cells from the Kidney
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Cited by 26 •
2014
DNabT cells are rare among peripheral T cells; however, they are abundant in certain non-lymphoid tissues. Difficulty of isolating DN T cells from non-lymphoid tissue hinders their functional analysis despite increasing recognized pathophysiologic significance. We describe a novel protocol for isolation of highly purified DN T cells from murine kidney.
06:04
Transplantation of Cells Directly into the Kidney of Adult Zebrafish
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Cited by 10 •
2011
Cell transplantation is an essential technique for studying tissue regeneration and for developing cell-based therapies of disease. We demonstrate here a microsurgical technique that permits the transplantation of genetically labeled cells directly into the kidney of adult zebrafish fish.
03:00
Extracting Intact Kidney from Mouse: A Technique to Obtain Kidney Without Renal Capsule from Murine Models
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2023
In this video, we describe the procedure for kidney extraction from the retroperitoneal space of the mouse. The extracted kidney can further be microdissected to obtain an intact kidney without a renal capsule and the surrounding fat layer.
09:15
Reliable and High Efficiency Extraction of Kidney Immune Cells
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Cited by 12 •
2016
Techniques that are reliable and efficient for the isolation of kidney immune cells are needed for downstream applications. This requires surface antibody labeling of a small number of kidney immune cells. Herein, we describe a concise method for isolation of kidney immune cells that seemingly achieves this goal.
FAQs
Polarity assigns different functions to the apical and basolateral surfaces of a cultured epithelial layer. This organization allows researchers to examine directional movement, such as uptake from one side and secretion or transfer toward the other. Preserving these distinct surfaces is therefore important when interpreting absorption, renal clearance, or drug-handling results from intestinal and renal models.
Tight junctions regulate paracellular passage, meaning movement between neighboring cells, and help maintain the selectivity of the epithelial barrier. Membrane transporters and channels instead control movement across cell membranes, including the uptake, secretion, and transfer of ions, nutrients, and drugs. Studying both mechanisms distinguishes barrier leakage from regulated cellular transport.
The two cell types represent different stages of how substances may be handled across organs. Intestinal models help address absorption, whereas renal models support investigation of clearance and toxicity. Comparing their responses can reveal organ-specific pharmacological effects and clarify how a treatment may influence more than one epithelial tissue during systemic exposure.
Researchers culture intestinal epithelial or renal cells under conditions that allow them to form polarized layers with separate apical and basolateral surfaces. They can then examine barrier regulation, membrane-mediated movement, or responses to injury and treatment. When interactions between tissues are important, organ-on-chip systems can connect intestinal and kidney compartments for coordinated analysis.
These models can provide information about selective barrier behavior, paracellular passage, and the movement of ions, nutrients, or drugs. They also support evaluation of absorption, renal clearance, drug toxicity, and cellular responses to injury or treatment. The resulting comparisons help connect transport behavior with pharmacological effects and epithelial physiology.
Connected organ-on-chip systems are useful when researchers need to examine communication or sequential responses between intestinal and renal tissue compartments. Rather than studying each cell type in isolation, these systems support investigation of gut–kidney interactions and systemic disease mechanisms. They can also help assess how treatment-related effects observed in one compartment relate to responses in the other.