Plasma Depletion
Plasma Depletion - Related Videos
06:17
Depletion of Specific Cell Populations by Complement Depletion
0 Views •
Cited by 15 •
2010
To effectively study the function of immune cell populations their purification is often required. Complement depletion is a fast and inexpensive technique for the isolation of immune cell populations with high purity.
07:55
Ground State Depletion Super-resolution Imaging in Mammalian Cells
0 Views •
Cited by 16 •
2017
This article outlines a protocol for the detection of one or more plasma and/or intracellular membrane proteins using Ground State Depletion (GSD) super-resolution microscopy in mammalian cells. Here, we discuss the benefits and considerations of using such approaches for the visualization and quantification of cellular proteins.
08:50
Depletion and Reconstitution of Macrophages in Mice
0 Views •
Cited by 118 •
2012
Macrophages play a central role in homeostasis and pathology in many tissues. The protocol presented here describes methods for depleting macrophages in vivo, deriving polarized macrophages from bone marrow aspirates, and adoptively transferring macrophages into mice. These techniques allow determination of the role that polarized macrophages play in health and disease.
08:42
How to Ignite an Atmospheric Pressure Microwave Plasma Torch without Any Additional Igniters
0 Views •
Cited by 26 •
2015
This movie shows how an atmospheric plasma torch can be ignited by microwaves with no additional igniters and provides a stable and continuous plasma operation suitable for plenty of applications.
08:36
An Atmospheric Pressure Plasma Setup to Investigate the Reactive Species Formation
0 Views •
Cited by 20 •
2016
An experimental setup was created for the helium-operated kHz frequency plasma jet. The setup includes a cage for the plasma power supply and jet and an in-house built reactor to monitor plasma-induced reactive species without the interference of the ambient atmosphere.
FAQs
Centrifugation separates blood components according to density, producing a plasma supernatant above more concentrated cellular material. This arrangement makes the liquid fraction accessible for removal without discarding the red blood cells, white blood cells, or platelets that collect below it. The resulting preparation supports analyses in which cellular elements must be examined with less soluble-material interference.
Removing plasma lowers the contribution of soluble plasma proteins and electrolytes to the sample, helping researchers study cellular behavior under more defined conditions. That compositional change can improve consistency between preparations and reduce background effects in laboratory assays. The purpose is not simply to concentrate cells, but to control which surrounding chemical components remain during subsequent biological analysis.
Filtration or washing can supplement centrifugation when researchers need additional separation beyond removal of the supernatant. Centrifugation primarily uses density to partition liquid and cellular material, whereas these added steps provide alternative or complementary ways to reduce unwanted plasma-associated material. The selected combination depends on whether the priority is recovering a cell-rich fraction, lowering interference, or achieving consistent composition.
Blood is first processed so its components can separate, typically by centrifugation. After distinct layers form, the plasma supernatant is removed while the concentrated cellular fraction is retained. If the preparation requires further refinement, filtration or washing can follow. Careful handling of these stages determines whether the final sample remains cell-rich and sufficiently reduced in soluble components for the planned assay.
The retained fraction may contain red blood cells, white blood cells, or platelets, depending on the sample and the intended analysis. Keeping these cellular elements concentrated allows investigators to examine cell behavior without the original plasma environment dominating the preparation. This is particularly useful when the assay requires a consistent cellular sample rather than an unchanged whole-blood composition.
In hematology and cell biology, plasma depletion helps prepare samples whose cellular content can be examined under controlled conditions. Transfusion research can also use the approach when separating soluble plasma constituents from blood cells is relevant to the experimental design. More broadly, it supports laboratory assays that require consistent sample composition, making comparisons across analyses less affected by variable plasma content.