This article outlines a protocol for the detection of one or more plasma and/or intracellular membrane proteins using Ground State Depletion (GSD) super-resolution microscopy in mammalian cells. Here, we discuss the benefits and considerations of using such approaches for the visualization and quantification of cellular proteins.
Dixon, R. E., Vivas, O., Hannigan, K. I., Dickson, E. J. Ground State Depletion Super-resolution Imaging in Mammalian Cells. J. Vis. Exp. (129), e56239, doi:10.3791/56239 (2017).