7,195 Views
•
07:55 min
•
November 05, 2017
DOI:
Summary
Automatically generated
This article outlines a protocol for the detection of one or more plasma and/or intracellular membrane proteins using Ground State Depletion (GSD) super-resolution microscopy in mammalian cells. Here, we discuss the benefits and considerations of using such approaches for the visualization and quantification of cellular proteins.
Related Videos
15:00
Long-term Imaging Mammalian Cells using Wide-Field Microscopy
Related Videos
5260 Views
07:49
Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
Related Videos
8233 Views
09:56
Super-resolution Imaging of the Natural Killer Cell Immunological Synapse on a Glass-supported Planar Lipid Bilayer
Related Videos
12388 Views
09:30
Super-resolution Imaging of Neuronal Dense-core Vesicles
Related Videos
9704 Views
10:41
Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
Related Videos
2152 Views
08:47
Super-resolution Imaging of the Bacterial Division Machinery
Related Videos
11805 Views
14:02
Super-Resolution Imaging to Study Co-Localization of Proteins and Synaptic Markers in Primary Neurons
Related Videos
5772 Views
12:44
Super-resolution Imaging of the Cytokinetic Z Ring in Live Bacteria Using Fast 3D-Structured Illumination Microscopy (f3D-SIM)
Related Videos
19965 Views
06:50
Super-Resolution Live Cell Imaging of Subcellular Structures
Related Videos
4777 Views
09:14
Super-Resolution Microscopy of the Synaptonemal Complex Within the Caenorhabditis elegans Germline
Related Videos
2521 Views
Read Article
Cite this Article
Dixon, R. E., Vivas, O., Hannigan, K. I., Dickson, E. J. Ground State Depletion Super-resolution Imaging in Mammalian Cells. J. Vis. Exp. (129), e56239, doi:10.3791/56239 (2017).
Copy