Tissue Expansion

Tissue expansion is a reconstructive technique that gradually increases the amount of available skin and soft tissue, helping restore form after cancer-related surgery. A silicone expander placed beneath the skin is incrementally filled, commonly with saline, creating controlled mechanical tension that stimulates tissue stretching, cellular proliferation, and remodeling over time. In oncology, the method supports breast reconstruction and repair of defects after tumor removal while using tissue that matches the patient’s color and texture. Outcomes depend on expansion rate, tissue quality, surgical planning, and management of complications such as infection, pain, or expander failure.

Tissue Expansion - Related Videos

Research

JoVE EoE - Immune Systems and Components

Natural Killer Cell Isolation from Liver Tissue for Selective Expansion

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2025

This video demonstrates the isolation of lymphocytes, including natural killer cells or NK cells, from human liver tissue for selective expansion. Enzymatic and mechanical digestion, followed by density gradient separation, promotes lymphocyte isolation, including NK cells. The collected lymphocytes can be further used for the selective expansion of NK cells.

Formation and Expansion of Neurospheres from a Hippocampal Tissue

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2025

This video demonstrates the process of neurosphere formation and their expansion from hippocampal tissue. Neural stem and progenitor cells from the postnatal mouse dentate gyrus tissue proliferate and form primary neurospheres, which are then reseeded to form secondary neurospheres.

Expansion Pathology: A Method for High-resolution Optical Imaging of Clinical Tissue Samples

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2023

In this video, we describe a method called expansion pathology, a variant of expansion microscopy, wherein the clinical tissue specimens are chemically expanded using a polymer network for microscopic examination of nanoscale structures.

Research

JoVE Journal - Developmental Biology
Free Sample

Isolation and Expansion of Mesenchymal Stem/Stromal Cells Derived from Human Placenta Tissue

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Cited by 91 •

2016

Herein we describe methods for the dissection of fetal and maternal tissues from human term placenta, followed by isolation and expansion of mesenchymal stem/stromal cells (MSC) from these tissues.

Education

JoVE Science Education - Engineering

Analysis of Thermal Expansion via Dilatometry

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2023

Source: J. Jacob Chavez, Ryan T. Davis, and Taylor D. Sparks, Department of Materials Science and Engineering, The University of Utah, Salt Lake City, UT Thermal expansion is extremely important when considering which materials will be used in systems that experience fluctuations in temperature. A high or low thermal expansion in a material may or may not be desirable, depending on the application. For instance, in a common liquid thermometer, a material with a high thermal expansion would be...

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