Anhydrous Dmso
Anhydrous Dmso - Related Videos
10:17
Preparing Anhydrous Reagents and Equipment
0 Views •
2023
Source: Laboratory of Dr. Dana Lashley - College of William and Mary Demonstrated by: Timothy Beck and Lucas Arney Many reactions in organic chemistry are moisture-sensitive and must be carried out under careful exclusion of water. In these cases the reagents have a high affinity to react with water from the atmosphere and if left exposed the desired reaction will not take place or give poor yields, because the reactants are chemically altered. In order to prevent undesired reactions with H2O...
02:18
Enhancing Stem Cell Differentiation with a Transient DMSO Treatment
0 Views •
2025
This video demonstrates the method for enhancing stem cell differentiation with transient DMSO treatment, which arrests cells in the G1 phase. Post-treatment, an ectodermal differentiation medium with BMP and TGF-β inhibitors is added to promote neural precursor formation.
06:55
Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation
0 Views •
Cited by 11 •
2019
Generating differentiated cell types from human pluripotent stem cells (hPSCs) holds great therapeutic promise but remains challenging. PSCs often exhibit an inherent inability to differentiate even when stimulated with a proper set of signals. Described here is a simple tool to enhance multilineage differentiation across a variety of PSC lines.
![Microwave-assisted One-pot Synthesis of <em>N</em>-succinimidyl-4-[<sup>18</sup>F]fluorobenzoate ([<sup>18</sup>F]SFB)](/_next/image?url=https%3A%2F%2Fcloudfront.jove.com%2FCDNSource%2Fteasers%2F2755.jpg%3Fv%3D2026-09-01%252004%253A39%253A14&w=750&q=75)
08:33
Microwave-assisted One-pot Synthesis of N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB)
0 Views •
Cited by 15 •
2011
A facile, one-pot synthesis of N-succinimidyl-4-[18F]fluorobenzoate ([18F]SFB) was developed based on a non-aqueous, three-step radiochemical process. Using microwave heating, the entire procedure can be completed in less than 30 min, or 60 min with further purification by preparative HPLC. The decay-corrected radiochemical yields (RCYs) were 35-5% (n > 30).
10:53
Anionic Polymerization of an Amphiphilic Copolymer for Preparation of Block Copolymer Micelles Stabilized by π-π Stacking Interactions
0 Views •
Cited by 2 •
2016
The key steps of living anionic polymerization of phenyl glycidyl ether (PheGE) on methoxy-polyethylene glycol (mPEG-b-PPheGE) are described. The resulting block copolymer micelles (BCMs) were loaded with doxorubicin 14% (wt%) and sustained release of drug over 4 days under physiologically relevant conditions was obtained.
FAQs
The strong polarization of DMSO's sulfur-oxygen bond gives the solvent high solvating power for many ionic species. At the same time, its lack of a readily exchangeable proton avoids introducing a proton source into reactions. This combination helps support nucleophilic substitution and other transformations in which ion solvation and controlled proton availability influence chemical behavior.
Trace water can hydrolyze moisture-sensitive reagents or compounds, promote proton-transfer side reactions, and alter the effective concentration of dissolved reagents. Those changes can shift reaction behavior and reduce reproducibility. Keeping the solvent water-free therefore matters not merely for solvent purity, but for preserving the intended chemical environment during synthesis or analytical sample preparation.
The high boiling point allows anhydrous DMSO to support reactions that require elevated temperatures without rapid solvent loss. This property can help maintain the intended solvent environment during heating. However, the same characteristic makes solvent removal more difficult after the reaction, so workup may require specialized methods rather than simple evaporation.
In synthesis, anhydrous DMSO provides a controlled medium for reactions affected by water, including nucleophilic substitution and other transformations that depend on limited proton availability. Its ability to solvate ionic species can support the desired chemical process, while water exclusion reduces hydrolysis and proton-transfer side reactions that could otherwise change the reaction outcome.
Analytical sample preparation benefits from anhydrous DMSO when water could alter a compound, reagent concentration, or measured sample composition. Removing that source of variation helps preserve the intended chemical state before analysis. The solvent's high solvating power also supports preparation of samples containing ionic species, provided the procedure requires a water-free environment.
Chemists should consider anhydrous DMSO when handling compounds or reagents whose behavior may change through hydrolysis or proton-transfer reactions. A water-free solvent helps maintain controlled conditions and improves reproducibility. This is especially relevant when the experiment depends on consistent reagent concentration, stable compound composition, or reaction performance that would otherwise be affected by moisture.