Dmso Cryoprotectant
Dmso Cryoprotectant - Related Videos
02:18
Enhancing Stem Cell Differentiation with a Transient DMSO Treatment
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2025
This video demonstrates the method for enhancing stem cell differentiation with transient DMSO treatment, which arrests cells in the G1 phase. Post-treatment, an ectodermal differentiation medium with BMP and TGF-β inhibitors is added to promote neural precursor formation.
06:55
Transient Treatment of Human Pluripotent Stem Cells with DMSO to Promote Differentiation
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Cited by 11 •
2019
Generating differentiated cell types from human pluripotent stem cells (hPSCs) holds great therapeutic promise but remains challenging. PSCs often exhibit an inherent inability to differentiate even when stimulated with a proper set of signals. Described here is a simple tool to enhance multilineage differentiation across a variety of PSC lines.
03:15
Rabbit Embryo Vitrification Using Cryogenic Straw: An Ultra-Rapid Cooling Technique to Preserve Rabbit Embryos for Long-term Storage
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2025
In this video, we describe the procedure for vitrification of harvested rabbit embryos at desired development stage using ethylene glycol and dimethyl sulfoxide as cryoprotectants and cryogenic straw as the carrier system. The vitrified embryos have high survival rate and can be stored for an extended period until use.
04:24
Vitrification-based Cryopreservation: An Ultra-rapid Cooling Technique to Preserve Biological Specimens at Very Low Temperatures for Long-term Storage
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2023
In this video, we demonstrate an ultra-rapid cryo vitrification method for preserving biological samples. The method entails the use of a combination of cryoprotectants to protect the biological tissue from freezing damage by preventing intracellular ice crystal formation.
02:35
Long-Term Cryopreservation of Cyanobacterial Strains Using Glycerol or Dimethyl Sulfoxide
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2025
Source: Lea-Smith, D. J., et al. Generation of Marked and Markerless Mutants in Model Cyanobacterial Species. J. Vis. Exp. (2016)This video demonstrates the cryopreservation of cyanobacterial strains by combining concentrated cell suspensions with glycerol or DMSO and storing them at low temperatures, enabling long-term maintenance of viable cultures for photosynthesis and genetic studies.
FAQs
These variables must be balanced because DMSO protection is not constant under all conditions. Concentration influences how effectively the solution limits freezing-related damage, while temperature and exposure time affect how cells experience the treatment. An appropriate protocol therefore considers all three together rather than treating DMSO concentration as an independent setting. This balance helps preserve viability during later recovery.
DMSO can cross cell membranes, allowing its protective action to occur within the cellular environment rather than only outside the cell. This permeability helps moderate the effects of water movement as temperature changes and reduces osmotic stress during cooling and thawing. The mechanism is especially relevant for developmental materials whose survival depends on maintaining cellular integrity for subsequent culture or analysis.
Protection must address injury in both directions of the temperature change. During cooling, DMSO helps reduce ice formation, while during thawing its presence helps limit osmotic stress as water shifts across cell membranes. Considering both phases is important because a sample that survives freezing may still lose viability during recovery if water movement and associated stress are not adequately controlled.
A protocol should specify the DMSO concentration, the temperature conditions, and the duration of cellular exposure, because each can alter protection. It should also address both cooling and thawing rather than focusing only on storage. These parameters are evaluated in relation to the biological material, since embryos, gametes, stem cells, and other tissues may differ in their response and recovery.
Developmental biology protocols use DMSO-based cryopreservation to maintain embryos, gametes, stem cells, and other valuable biological materials for later work. Preserved samples can support subsequent culture and analysis without requiring all material to be used immediately. This approach gives researchers access to stored developmental resources and helps maintain continuity across experiments.
Reliable preservation can reduce the loss of cell viability and make developmental studies easier to repeat. Researchers can store valuable material, recover it for later culture, and compare results across experiments conducted at different times. Maintaining embryos, gametes, stem cells, and related resources also supports the retention of genetic material for continued investigation rather than requiring fresh samples for every study.