Embryonic Transplantation
Embryonic Transplantation - Related Videos
18:57
Tissue Targeted Embryonic Chimeras: Zebrafish Gastrula Cell Transplantation
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Cited by 12 •
2009
Zebrafish cell transplantation enables the combination of genetics and embryology to generate tissue specific chimeras. This video demonstrates gastrula staged cell transplantations that have allowed our lab to investigate the roles of astroglial populations and specific guidance cues during commissure formation in the forebrain.
10:51
A System for ex vivo Culturing of Embryonic Pancreas
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Cited by 23 •
2012
Here, we describe a method for isolation, culture and manipulation of mouse embryonic pancreas. This represents an excellent ex vivo system for studying various aspects of pancreatic development, including morphogenesis, differentiation and growth. Pancreatic bud explants can be cultured for several days and used in a range of different applications, including whole-mount immunofluorescence and live imaging.
06:21
Transplantation Studies
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2023
Many developmental biologists are interested in the molecular signals and cellular interactions that induce a group of cells to develop into a particular tissue. To investigate this, scientists can use a classic technique known as transplantation, which involves tissue from a donor embryo being excised and grafted into a host embryo. By observing how transplanted tissues develop in host environments, scientists have started to dissect the molecular pathways underlying development. In this...
06:08
High-resolution Cell Transplantation in Embryonic and Larval Zebrafish
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2024
Here we present a protocol to transplant cells with high spatial and temporal resolution in zebrafish embryos and larvae at any stage between at least 1 and 7 days post fertilization.
08:15
Cervical Heterotopic Mouse Heart Transplantation using End-to-side Anastomosis Technique to Study Transplant Immunology
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2026
This protocol presents a simplified, end-to-side suture anastomosis technique for murine cervical heart transplantation, providing a robust and reproducible model for studying transplant immunology.
FAQs
The method compares what donor cells do after relocation with what they would be expected to do in their original setting. If transplanted cells retain their developmental behavior, intrinsic properties may be important. If their fate or organization changes in the new position or host, signals from neighboring tissues may influence them. This comparison helps separate cell-autonomous programs from environmental control.
Transplantation can reveal whether a particular embryonic region organizes development in surrounding tissue. When donor tissue is placed elsewhere, researchers can examine whether it influences nearby cells and alters pattern formation. Such results provide evidence for developmental induction, in which one cell population affects the fate or organization of another, helping explain how body axes and organs are established.
Relocating embryonic tissue exposes it to a different local environment, including signals from neighboring cell populations. Researchers can then assess whether the tissue follows its original developmental tendencies or responds to the new surroundings. Differences in cell fate, pattern formation, or tissue organization indicate that position and local interactions contribute to developmental control rather than acting as incidental features of the experiment.
The approach tests whether one cell population can influence the behavior of another after the populations are placed in a new arrangement. Observing changes in fate or tissue organization can identify instructive interactions between neighboring groups. This is especially useful for studying induction and for determining how coordinated signals contribute to specialized tissues, organs, and larger embryonic patterns.
A typical experiment begins by selecting and carefully isolating an embryo, embryonic tissue, or specific cell population. The donor material is then transferred to a different position or host. Researchers subsequently assess its developmental fate, the organization of the transplanted tissue, and effects on surrounding cells. These observations are compared with the donor's expected behavior to interpret developmental control.
The transferable material may be an entire embryo, a defined embryonic tissue, or a specific population of cells. This range allows investigators to ask questions at different levels of organization, from broad pattern formation to the behavior of a particular cell group. The selected material should match the developmental question and the tissue interactions that researchers intend to examine.
Post-transplant analysis focuses on cell fate, pattern formation, and tissue organization. Researchers determine whether donor material preserves its intrinsic developmental properties, changes in response to the new environment, or influences nearby cells. These outcomes can provide evidence for organizers, induction, and the relative contributions of intrinsic programs and external signals during embryonic development.
This experimental strategy provides a direct way to test how developmental decisions are controlled rather than only observing where tissues normally arise. By changing the relationship between donor material and its surroundings, researchers can investigate mechanisms that establish body axes, form organs, and produce specialized tissues. It therefore connects local cell interactions with larger patterns of embryonic organization.