Luminal Cells
Luminal Cells - Related Videos
09:59
Transfer of Mammary Gland-forming Ability Between Mammary Basal Epithelial Cells and Mammary Luminal Cells via Extracellular Vesicles/Exosomes
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Cited by 8 •
2017
This protocol describes methods for purifying, quantitating, and characterizing extracellular vesicles (EVs)/exosomes from non-adherent/mesenchymal mammary epithelial cells and for using them to transfer mammary gland-forming ability to luminal mammary epithelial cells. EVs/exosomes derived from stem-like mammary epithelial cells can transfer this cell property to cells that ingest the EVs/exosomes.
08:24
Profiling Luminal pH in Three-Dimensional Gastrointestinal Organoids Using Microelectrodes
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Cited by 2 •
2024
The present protocol describes pH measurements in human tissue-derived gastric organoids using microelectrodes for spatiotemporal characterization of intraluminal physiology.
10:21
Studying Murine Small Bowel Mechanosensing of Luminal Particulates
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Cited by 3 •
2022
To study how the small bowel handles particulates of varying sizes, we have modified an established in vivo method to determine small bowel transit.
09:17
In Vivo Luminal Measurement of Distension-Evoked Urothelial ATP Release in Rodents
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Cited by 1 •
2022
This protocol describes the procedure for measuring ATP concentrations in the lumen of the bladder in an anesthetized rodent.
08:40
Improved Method for the Preparation of a Human Cell-based, Contact Model of the Blood-Brain Barrier
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Cited by 16 •
2013
Establishment of human models of the blood-brain barrier (BBB) can benefit research into brain conditions associated with BBB failure. We describe here an improved technique for preparation of a contact BBB model, which permits coculturing of human astrocytes and brain endothelial cells on the opposite sides of a porous membrane.
FAQs
Apicobasal polarity gives cells distinct positional organization along the tissue-fluid axis. It helps coordinate the placement of adherens and tight junctions, which support epithelial continuity as a lumen forms and is maintained. In developmental studies, polarity is therefore examined as a structural prerequisite for organized epithelial architecture.
Adherens and tight junctions help neighboring cells organize into a continuous epithelial boundary. Their coordinated arrangement supports the separation and maintenance of the luminal space while cells mature. Developmental defects affecting this organization can be interpreted through changes in tissue architecture, because junctional structure is linked to both epithelial continuity and cellular polarity.
Progenitor cells contribute to lumen formation through coordinated division and differentiation. The orientation of division helps arrange new cells within the developing epithelium, while differentiation establishes the mature cellular properties needed for organized tissue structure. Studying both processes reveals how a developing cell population becomes a continuous, specialized epithelial lining rather than an unorganized group of cells.
Transport across the epithelium can influence both the size and the contents of a lumen. This means lumen development depends not only on how cells are positioned and connected, but also on coordinated movement across the epithelial layer. Developmental analyses can therefore relate changes in luminal dimensions or contents to the functional maturation of the surrounding cells.
Developmental biology examines luminal cells by relating their polarity, junctional organization, division orientation, differentiation, and transport activity to tissue architecture. These features help explain how organs and glands form and mature. The approach is especially informative when comparing epithelial development in the mammary gland, kidney, and airway, where luminal organization is central to organ structure.
The mammary gland, kidney, and airway provide distinct developmental contexts for examining luminal organization. Across these systems, researchers can consider how epithelial cells establish polarity, maintain junctions, orient divisions, differentiate, and regulate lumen size or contents. Comparing these settings helps connect shared principles of epithelial development with organ-specific patterns of tissue formation and maturation.
Disrupted polarity or differentiation can indicate that epithelial cells are failing to organize or mature normally during development. Because these processes contribute to continuous tissue architecture and lumen maintenance, their disruption may help explain developmental abnormalities and disease-related changes. Luminal cell studies therefore connect cellular organization with broader outcomes in organ formation and epithelial maturation.