Local Derivative

A local derivative describes how a quantity changes at a specific point, providing the instantaneous rate of change rather than an average over a larger interval. It is obtained as the limit of a difference quotient as the change in the independent variable approaches zero, or equivalently as the slope of the function’s tangent at that point. In engineering, local derivatives support sensitivity analysis, system modeling, optimization, and control by showing how small changes in inputs affect outputs. They also enable local linearization, allowing complex nonlinear behavior to be approximated near an operating condition for design, prediction, and stability assessment.

Local Derivative - Related Videos

Research

JoVE Journal - Biology
Free Sample

Nano-fEM: Protein Localization Using Photo-activated Localization Microscopy and Electron Microscopy

0 Views •

Cited by 14 •

2012

We describe a method to localize fluorescently tagged proteins in electron micrographs. Fluorescence is first localized using photo-activated localization microscopy on ultrathin sections. These images are then aligned to electron micrographs of the same section.

Research

JoVE Journal - Immunology and Infection

Bone Marrow-derived Macrophage Production

0 Views •

Cited by 205 •

2013

Macrophages have long been recognized as a critical component of the innate and adaptive immune responses. The recent explosion of knowledge pertaining to evolutionary, genetic, and biochemical aspects of the interaction between macrophages and microbes has renewed scientific attention to macrophages. This article describes a method to differentiate macrophages from mouse bone marrow.

Analyzing the α-Actinin Network in Human iPSC-Derived Cardiomyocytes Using Single Molecule Localization Microscopy

0 Views •

Cited by 1 •

2020

The formation of a proper sarcomere network is important for the maturation of iPSC-derived cardiomyocytes. We present a super resolution-based approach that allows for the quantitative evaluation of the structural maturation of stem cell derived cardiomyocytes, to improve culture conditions promoting cardiac development.

Research

JoVE Journal - Biology
Free Sample

Heterokaryon Technique for Analysis of Cell Type-specific Localization

0 Views •

Cited by 1 •

2011

A flexible and efficient method for the characterization of cell type-specific protein localization and nucleocytoplasmic shuttling is described. This heterokaryon approach uses fluorescently-labeled fusion proteins to image protein localizations after cell fusion. The protocol is amenable to steady-state localizations or more dynamic determinations based on live cell imaging.

Visualizing RNA Localization in Xenopus Oocytes

0 Views •

Cited by 5 •

2010

Visualization of in vivo RNA transport is accomplished by microinjection of fluorescently labeled RNA transcripts into Xenopus oocytes, followed by confocal microscopy.

View All Results

FAQs

Related Topics