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07:01
Frequency Mixing Magnetic Detection Scanner for Imaging Magnetic Particles in Planar Samples
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Cited by 5 •
2016
A scanner for imaging magnetic particles in planar samples was developed using the planar frequency mixing magnetic detection technique. The magnetic intermodulation product response from the nonlinear nonhysteretic magnetization of the particles is recorded upon a two-frequency excitation. It can be used to take 2D images of thin biological samples.
08:25
Using Flatbed Scanners to Collect High-resolution Time-lapsed Images of the Arabidopsis Root Gravitropic Response
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Cited by 6 •
2014
This protocol describes a process for rapid collection of images of Arabidopsis seedlings responding to a gravity stimulus using commercially-available flatbed scanners. The method allows for inexpensive, high-volume capture of high-resolution images amenable for downstream analysis algorithms.
08:29
Expression Profiling with Microarrays
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2023
Microarrays are important tools for profiling gene expression, and are based on complementary binding between probes that are attached to glass chips and nucleic acids derived from samples. Using these arrays, scientists can simultaneously evaluate the expression of thousands of genes. In addition, the expression profiles of different cells or tissue types can be compared, allowing researchers to deduce how the expression of different genes change during biological processes, and thus gain...
07:13
Using Topographic Maps to Generate Topographic Profiles
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2023
Source: Laboratory of Alan Lester - University of Colorado Boulder Topographic maps are "plan-view" representations of Earth's three-dimensional surface. They are a standard type of map-view that provides an overhead, or aerial, perspective. Among the defining features of a topographic map are the contour lines that indicate locations of constant elevation. The elevation interval between the contour lines is dependent on the level of detail provided by the map and the kind of topography present.
09:16
Eukaryotic Polyribosome Profile Analysis
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Cited by 51 •
2010
This article describes a protocol for the extraction of translating ribosomes from eukaryotic cells. Once extracted, ribosomes are separated into monosomes and polyribosomes by sucrose gradient fractionation to allow different ribosomal populations to be analyzed. As such, this method is the gold standard for examining the regulation of translation.
FAQs
The reconstruction process uses reflected light and imaging data to infer a cross-sectional shape. In triangulation, the system compares the projected laser line with its observed position, allowing geometry to be calculated as the scanner or object moves. This converts successive profiles into measurement information for dimensional inspection and three-dimensional modeling.
A laser line or structured light creates a measurable pattern on the surface rather than relying on contact. Reflected light is captured by an imaging sensor, and changes in the pattern reveal surface geometry. This optical arrangement supports rapid, non-contact acquisition, which is useful when engineers need repeated inspection without physically touching the component.
Point-based measurement records individual locations, whereas profile scanning captures a cross-sectional shape containing broader geometric information. As the object or scanner moves, successive profiles can describe more of the component’s surface. This makes the approach suitable for examining complex geometry, identifying dimensional deviations, and supporting three-dimensional models rather than evaluating isolated points alone.
A typical workflow projects a laser line or structured-light pattern onto the component, records the reflected light with an imaging sensor, and uses triangulation or image information to reconstruct a profile. The scanner or object then moves so additional cross-sections can be captured. Engineers use the resulting data for inspection, modeling, or process monitoring.
Engineers may select profile scanning for dimensional inspection, quality control, reverse engineering, or automated manufacturing. The method is especially relevant when rapid, non-contact measurements are needed to check tolerances or identify defects. In production environments, repeated profiles can also help monitor the process and improve consistency in components with complex shapes.
Profile-scanning data can show the measured shape, dimensions, and surface geometry of a component. Engineers can use these results to identify defects, verify tolerances, and build three-dimensional models for reverse engineering. When collected during production, the measurements also provide information for monitoring manufacturing performance and maintaining consistency across complex components.