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JoVE Journal
Biology

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Eukaryotic Polyribosome Profile Analysis
 

Eukaryotic Polyribosome Profile Analysis

Article doi: 10.3791/1948
June 15th, 2010

Summary June 15th, 2010

This article describes a protocol for the extraction of translating ribosomes from eukaryotic cells. Once extracted, ribosomes are separated into monosomes and polyribosomes by sucrose gradient fractionation to allow different ribosomal populations to be analyzed. As such, this method is the gold standard for examining the regulation of translation.

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