Double-ended Needle
Double-ended Needle - Related Videos
09:51
Intraosseous Needle Placement
0 Views •
2023
Source: Julianna Jung, MD, FACEP, Associate Professor of Emergency Medicine, The Johns Hopkins University School of Medicine, Maryland, USA For unstable patients requiring urgent administration of medications, fluids, or blood products, establishing vascular access quickly is essential. However, there are many factors that can complicate placement of a peripheral intravenous cannula (PIV), and it is extremely common for PIV attempts to fail. PIV placement may be technically challenging in small...
08:25
Needle Thoracostomy
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2015
Source: Rachel Liu, BAO, MBBCh, Emergency Medicine, Yale School of Medicine, New Haven, Connecticut, USA A tension pneumothorax is a life-threatening situation in which excess air is introduced into the pleural space surrounding the lung, either through trauma to the chest cavity or as a spontaneous leak of air from the lung itself. Air trapped within the pleural space causes separation of the lung from the chest wall, disrupting normal breathing mechanisms. Pneumothorax may be small without...
10:06
Personalized Needles for Microinjections in the Rodent Brain
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Cited by 3 •
2018
We describe here a protocol for microinjection in the rodent brain that uses quartz needles. These needles do not produce detectable tissue damage and ensure reliable delivery even in deep regions. Moreover, they can be adapted to research needs by personalized designs and can be re-used.
02:04
Fixing Double-strand Breaks
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2020
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
06:00
Wet Beveling of Microinjection Needles Utilizing Constant Air Pressure for Feedback on Needle Opening
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2024
This protocol describes the assembly of a pneumatic system for the delivery of pressurized air to a needle during the process of needle beveling. The protocol further describes the beveling process for creating sharp microinjection needles and how to gauge the relative opening size of the needle.
FAQs
The two ends can perform complementary roles within one procedure. One end may cross a septum, membrane, or other barrier, while the opposite end connects to equipment or supports delivery or sampling. This arrangement allows a protocol to coordinate access and material movement more directly, which can improve control during biological sample handling or inoculation.
By combining barrier access with attachment, delivery, or sampling functions, the device can reduce the need to reposition materials or introduce separate handling components. Fewer handling steps may limit opportunities for unintended contact or contamination. In infection and immunology workflows, this benefit supports more consistent manipulation of biological materials when the device matches the approved experimental protocol.
Performance depends on how the two ends are configured for the intended task. One point or connector must provide suitable access through the relevant barrier, while the other must support the required attachment, delivery, or collection step. Consequently, the device design and protocol must be considered together rather than treating all double-ended needles as interchangeable.
Its paired-end configuration can standardize how biological materials enter or leave a system. Using the same controlled access and transfer arrangement across experiments may reduce variation caused by extra manipulation. This is relevant when preparing samples, performing inoculation procedures, or collecting material for immune-related or infection-focused investigations that require comparable experimental handling.
A general workflow begins by matching the device to the procedure and identifying which end will access the septum, membrane, or other barrier. The opposite end is then connected or positioned for delivery or sampling, after which the protocol directs material transfer or collection. The specific sequence remains dependent on the device and experimental requirements.
Researchers may select this device when a procedure requires controlled access through a barrier together with immediate delivery or sampling. In immunology and infection research, that combination can support preparation of biological samples and inoculation workflows while reducing unnecessary handling. Its value is greatest when the protocol requires precise, repeatable manipulation of immune-related or infectious materials.
The approach can support more reproducible preparation, introduction, and collection of biological materials. Its design helps organize access and transfer within a defined workflow, which may improve consistency between experimental runs and reduce handling-related contamination opportunities. These outcomes are useful for procedures involving inoculation, sample preparation, or collection in immunology and infection studies.