Human Lung Tissue
Human Lung Tissue - Related Videos
02:42
Imaging Bacterial Localization and Host Interaction in Human Lung Tissue
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2026
Source: Mills, B., et al., Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy. J. Vis. Exp. (2017)This video demonstrates real-time imaging of fluorescently labeled Staphylococcus aureus in suspension and lung tissue using fibered confocal fluorescence microscopy.
10:10
A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection
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Cited by 40 •
2015
Human tuberculosis infection is a complex process, which is difficult to model in vitro. Here we describe a novel 3D human lung tissue model that recapitulates the dynamics that occur during infection, including the migration of immune cells and early granuloma formation in a physiological environment.
09:24
Optical Screening of Novel Bacteria-specific Probes on Ex Vivo Human Lung Tissue by Confocal Laser Endomicroscopy
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Cited by 10 •
2017
This technique describes an efficient screening process for evaluating bacteria-specific optical imaging agents within ex vivo human lung tissue, by fibered confocal fluorescence microscopy for the rapid identification of small molecule chemical probe-candidates with translatable potential.
05:47
Generation of Human 3D Lung Tissue Cultures (3D-LTCs) for Disease Modeling
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Cited by 46 •
2019
Here, we present a protocol for the preparation of agarose-filled human precision-cut lung slices from resected patient tissue that are suitable for generating 3D lung tissue cultures to model human lung diseases in biological and biomedical studies.
07:40
Digital Analysis of Immunostaining of ZW10 Interacting Protein in Human Lung Tissues
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Cited by 2 •
2019
ZW10 interacting protein (ZWINT) participates in the mitotic spindle checkpoint and the pathogenesis of carcinoma. Here, we introduce a methodology of the immunostaining of ZWINT in human lung cancer tissues, followed by the digital scanning of whole slides and image analysis. This methodology can provide high-quality digital images and reliable results.
FAQs
Protection depends on coordinated barriers rather than a single cell type. Airway epithelium, mucus, and cilia help intercept and clear inhaled material, while alveolar cells, macrophages, and resident immune populations contribute recognition and responses within deeper regions. This layered organization allows the tissue to support gas exchange while limiting exposure of vulnerable areas to pathogens.
Resident immune populations provide local surveillance before inflammation recruits additional cells. When infectious agents are recognized, inflammatory signaling helps bring those cells into the affected tissue, strengthening the response. This signaling context is important for interpreting infection studies because it can be examined alongside tissue injury and subsequent repair rather than as an isolated immune event.
Human lung tissue is especially informative when researchers connect pathogen exposure with tissue-level consequences. Studies can examine how infection-associated inflammation relates to injury and repair, rather than measuring immune activity in isolation. This perspective is relevant to pneumonia and influenza, as well as chronic lung disease, where inflammatory responses may be studied in relation to ongoing tissue change.
Primary human lung tissue, organoids, and tissue-based models support research on host-pathogen interactions, respiratory inflammation, tissue injury, and repair. These model formats also provide platforms for evaluating vaccines, antimicrobial therapies, and immune-modulating treatments. Using tissue-centered systems keeps the investigation connected to the organized lung environment described in infectious and inflammatory disease studies.
These studies can connect infectious agents with barrier function, immune recognition, inflammatory signaling, tissue injury, and repair. Examining several processes within the same research framework helps clarify how respiratory infection affects both defense and tissue condition. The resulting information supports investigation of diseases including pneumonia and influenza, while also providing context for chronic lung disease research.
Primary tissue, organoids, and tissue-based models can support evaluation of vaccines, antimicrobial therapies, and immune-modulating treatments. Their value comes from allowing researchers to examine these interventions in relation to respiratory inflammation, host-pathogen interactions, tissue injury, and repair. This makes them relevant for studying both protective responses and tissue-level consequences associated with infection.