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Immunology and Infection
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A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection

A 3D Human Lung Tissue Model for Functional Studies on Mycobacterium tuberculosis Infection

DOI:
10.3791/53084-v

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10:10 min

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October 05, 2015

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, , , ,
1Department of Clinical and Experimental Medicine,Linköping University, 2Department of Medicine,Karolinska Institute

Chapters

  • 00:05Title
  • 01:13Fibroblast-collagen Matrix Continuous Culture
  • 03:35Immune and Lung Epithelial Cell Seeding
  • 05:423D Lung Tissue Model Air Exposure, Harvest and Mounting
  • 07:123D Model Visualization, Acquisition and Quantitative Analysis
  • 08:26Results: Representative 3D Visualization and Quantitative Tissue Model Analysis
  • 09:39Conclusion

Summary

Automatic Translation

Automatic Translation

Human tuberculosis infection is a complex process, which is difficult to model in vitro. Here we describe a novel 3D human lung tissue model that recapitulates the dynamics that occur during infection, including the migration of immune cells and early granuloma formation in a physiological environment.

Tags

Mycobacterium TuberculosisTuberculosisIn Vitro ModelGranulomaHost-pathogen InteractionsLung InfectionOrganotypic CultureQuantitative Analysis

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