Lymphoid Cell Isolation
Lymphoid Cell Isolation - Related Videos
04:39
In Vitro Generation of Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors
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2025
This video demonstrates an in vitro assay to generate plasmacytoid dendritic cells (pDCs) from common lymphoid progenitor cells (CLPs). Upon co-culturing purified CLPs with γ-irradiated feeder cells in the presence of Fms-like tyrosine kinase-3 ligand (FL), the CLPs differentiate into non-adherent pDCs, the presence of which is confirmed using flow cytometry.
01:18
Lymphoid Cells and Tissues
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2024
Lymphoid cells and tissues are integral to the immune system, which is crucial in maintaining our body's defense against harmful pathogens. They form the building blocks of lymphoid organs, which include the spleen, thymus, and lymph nodes. Lymphoid cells consist of various types of immune system cells. These include B and T lymphocytes, which are responsible for producing antibodies and killing infected cells, respectively. Dendritic cells act as messengers between the innate and adaptive...
09:02
Isolation of Uterine Innate Lymphoid Cells for Analysis by Flow Cytometry
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Cited by 5 •
2021
This is a method to isolate uterine lymphoid cells from both pregnant and non-pregnant mice. This method can be used for multiple downstream applications such as FACS phenotyping, cell sorting, functional assays, RNA-seq, and proteomics. The protocol here demonstrates how to phenotype group 1 uterine innate lymphoid cells by flow cytometry.
08:30
Isolation of Group 2 Innate Lymphoid Cells from Mouse Nasal Mucosa to Detect the Expression of CD226
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2022
Group 2 innate lymphoid cells (ILC2s), implicated in type 2 inflammation, mainly participate in response to helminth infection, allergic diseases, metabolic homeostasis, and tissue repair. In this study, a procedure to isolate ILC2s from murine nasal mucosa and detect the expression of CD226 is demonstrated.
08:18
In Vitro Generation of Murine Plasmacytoid Dendritic Cells from Common Lymphoid Progenitors using the AC-6 Feeder System
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Cited by 3 •
2015
In this study we present an in vitro culture system that can efficiently generate pDCs by co-culturing common lymphoid progenitors with AC-6 feeder cells in the presence of Flt3 ligand.
FAQs
Density-based separation works by centrifuging a cell suspension through a gradient so populations partition according to density. This step can reduce a mixture from blood or dissociated tissue to a fraction enriched for lymphoid cells before more selective enrichment. It provides a broad starting population for immunophenotyping or downstream sorting without relying on a single surface marker.
Antibody-based enrichment adds specificity by recognizing surface markers on target cells. Magnetic particles can capture or enrich cells labeled with those antibodies, whereas fluorescence-activated cell sorting uses fluorescent markers to identify and physically sort defined populations. Magnetic selection is suited to marker-based enrichment, while fluorescence-based sorting supports more detailed separation of lymphoid subsets.
Viability and population purity are central quality criteria because isolated cells must remain suitable for controlled study. Preserved viability supports functional assays, while defined purity helps connect observed immunophenotypes or responses to particular lymphoid subsets. These qualities are especially important when comparing immune activation, disease progression, or treatment responses in immunology and infection research.
A typical workflow starts with blood, a lymphoid organ, or infected tissue as the source material. Tissue samples undergo dissociation to release cells, followed by density-gradient centrifugation to separate populations. Researchers may then apply antibody-coupled magnetic particles or fluorescence-activated cell sorting to enrich cells by surface markers before using the preparation in downstream studies.
Isolated populations support several complementary analyses. Immunophenotyping characterizes lymphoid subsets, functional assays examine their activity, and pathogen-response studies assess reactions relevant to infection. The same preparations can also support analysis of cell-mediated immunity, allowing researchers to examine cellular behavior under controlled conditions rather than interpreting responses from an unseparated mixture.
Separating lymphoid subsets enables researchers to associate particular cells with immune activation, disease progression, or treatment responses. In infected tissues, the process can provide populations for pathogen-response studies and analysis of cell-mediated immunity. This subject-specific use depends on preserving both viability and population purity so measured responses remain attributable to the cells under investigation.