Modified Ldh Assay
Modified Ldh Assay - Related Videos
12:17
Analysis of Trunk Neural Crest Cell Migration using a Modified Zigmond Chamber Assay
0 Views •
Cited by 5 •
2012
An approach to analyze the migration of explanted cells (trunk neural crest cells) is described. This method is inexpensive, gentle, and capable of distinguishing chemotaxis from both chemokinesis and other influences on migratory polarity such as those derived from cell-cell interactions within the primary trunk neural crest cell culture.
04:12
Modified Yeast-One Hybrid Assay to Detect Heteromeric Protein Complex-DNA Interactions
0 Views •
2025
In this video, we demonstrate the modified yeast one-hybrid assay, which detects multiple proteins interacting with target DNA. The transformed yeast cells contain the target DNA sequence upstream of the reporter gene. Additionally, they express transcription factor proteins. When the proteins form complexes and bind to the target DNA, the reporter gene is expressed and can be detected enzymatically.
10:38
In Vivo Alkaline Comet Assay and Enzyme-modified Alkaline Comet Assay for Measuring DNA Strand Breaks and Oxidative DNA Damage in Rat Liver
0 Views •
Cited by 17 •
2016
The alkaline comet assay measures DNA strand breaks in eukaryotic cells. By adding an Endonuclease III or human 8-oxoguanine-DNA-N-glycosylase digestion step, the assay can efficiently detect oxidative DNA damage. We describe methods for using these assays to detect DNA damage in rat liver.
07:34
Modified Annexin V/Propidium Iodide Apoptosis Assay For Accurate Assessment of Cell Death
0 Views •
Cited by 543 •
2011
An accurate method for the assessment of cell death is described. The protocol improves upon conventional Annexin V/ propidium iodide (PI) protocols, which display up to 40% false- positive events in cell lines and primary cells from a broad range of animal models.
03:02
A Lactate Dehydrogenase Release Assay To Detect Macrophage Death Following Nigericin Exposure
0 Views •
2025
This video demonstrates the lactate dehydrogenase assay to detect inflammasome-mediated macrophage death. The macrophages are exposed to lipopolysaccharide for priming, followed by exposure to nigericin to cause inflammasome-induced cell death, which is determined by the lactate dehydrogenase assay.
FAQs
When plasma membranes become compromised, LDH escapes from the cells into the surrounding sample. The released enzyme then catalyzes reactions that produce a colored product. Greater color intensity corresponds to more LDH in the sample and therefore provides an estimate of membrane disruption. This relationship allows cytotoxicity to be assessed without directly observing every damaged cell.
A single assay format may not suit every cell type, infection model, or experimental design. Modifications can adapt the measurement to the biological system being studied and accommodate appropriate experimental controls. This flexibility helps investigators evaluate host-cell injury, immune-cell activity, or pathogen-associated damage under conditions relevant to their specific model.
The modified LDH assay generates a measurable color signal rather than requiring a radioactive label. That difference makes it useful when researchers want to assess cell damage through a nonradioactive readout. In infection and immunology studies, the colorimetric format can support comparisons among pathogen exposure, immune-cell activity, and treatment conditions while avoiding dependence on radioactive detection.
Researchers first establish the relevant cell, infection, immune, or treatment conditions and include the experimental controls needed for interpretation. They then measure LDH released into the sample through the assay’s enzyme-coupled color-generating reactions. Finally, the resulting color intensity is compared across samples to evaluate differences in membrane disruption or cytotoxicity.
Differences in signal indicate differences in the amount of LDH released and therefore in the extent of cell membrane disruption. In an infection model, this can reveal pathogen-associated host-cell injury; in an immune assay, it can indicate cytotoxic activity. Comparisons with suitable controls also help assess whether antimicrobial or immunomodulatory treatments alter cellular damage.
The assay supports several related investigations, including host-cell viability during infection, immune-cell cytotoxicity, pathogen-associated injury, and treatment effects. Researchers can adapt the format to the cell type or infection model and use the colorimetric outcome to compare experimental conditions. These measurements provide a shared readout for evaluating cellular damage across different biological contexts.