Gustatory Receptor Analysis
Gustatory Receptor Analysis - Related Videos
10:59
New Methods to Study Gustatory Coding
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Cited by 1 •
2017
We present three new methods to study gustatory coding. Using a simple animal, the moth Manduca sexta (Manduca), we describe a dissection protocol, the use of extracellular tetrodes to record the activity of multiple gustatory receptor neurons, and a system for delivering and monitoring precisely timed pulses of tastants.
10:57
RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)
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Cited by 19 •
2013
In this protocol, we describe two strategies that simultaneously suppress two genes (double gene knockdown) in honey bees. Then we present how to use the proboscis extension response (PER) assay to study the effect of double gene knockdown on honey bee gustatory perception.
12:02
High-throughput Analysis of Mammalian Olfactory Receptors: Measurement of Receptor Activation via Luciferase Activity
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Cited by 16 •
2014
Olfactory receptor activation patterns encode odor identity, but the lack of published data identifying odorant ligands for mammalian olfactory receptors hinders the development of a comprehensive model of odor coding. This protocol describes a method to facilitate high-throughput identification of olfactory receptor ligands and quantification of receptor activation.
07:48
Tracking Drug-induced Changes in Receptor Post-internalization Trafficking by Colocalizational Analysis
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Cited by 1 •
2015
Receptor trafficking modulates signaling and cell responsiveness to ligands and is, itself, responsive to cell conditions, including ligand-induced signaling. Here, we describe a powerful and flexible technique for quantitatively assessing drug-induced receptor trafficking using immunolabeling and colocalizational analysis.
07:30
A Novel Behavioral Assay to Investigate Gustatory Responses of Individual, Freely-moving Bumble Bees (Bombus terrestris)
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Cited by 21 •
2016
A novel behavioral assay is described for investigating the short term gustatory responses of the mouthparts of freely-moving bumble bees (Bombus terrestris) toward nutrients and toxins in solution.
FAQs
Gustatory receptor analysis separates receptor presence from receptor function by examining expression alongside ligand responsiveness. A receptor may be detected in a sample through gene-expression profiling, while binding or reporter measurements assess whether a microbial or host-derived compound activates it. This distinction helps investigators connect receptor distribution with specific chemical cues rather than treating expression as proof of signaling.
Activation can initiate G-protein signaling, followed by phospholipase C activity and release of calcium inside the cell. These events provide a mechanistic readout connecting ligand recognition to intracellular activity. Measuring this sequence is useful because calcium release and related downstream responses can indicate that receptor engagement has progressed beyond simple binding.
Gene-expression profiling identifies where receptor transcripts are present, whereas receptor-binding or reporter assays address ligand interaction and activation. Combining these measurements can reveal cases in which a receptor is expressed but not shown to respond to a tested compound, or in which a response can be linked to a particular ligand. This pairing strengthens interpretation of infection-related receptor studies.
A study can profile receptor gene expression, expose the relevant receptor system to candidate compounds, and measure binding or reporter activity. Investigators then examine signaling outputs, including G-protein-associated activity, phospholipase C activity, or intracellular calcium release. Comparing these measurements helps identify activated receptors and relate chemical detection to cellular responses.
Activation measurements can be connected to epithelial defense, immune-cell behavior, and broader host responses to infection. The key value is functional linkage: a microbial or host-derived compound is not merely cataloged, but evaluated for its capacity to stimulate receptor-associated signaling. This approach can help investigators investigate how chemical cues influence tissues or cells during infectious conditions.
These analyses can support biomarker discovery and the evaluation of receptor-targeted interventions. Expression data indicate which receptors warrant attention, while ligand and signaling measurements help determine whether those receptors respond to relevant compounds. In infection studies, integrating both types of evidence can prioritize receptor activities associated with epithelial or immune responses for further investigation.