Near-infrared Light Irradiation
Near-infrared Light Irradiation - Related Videos
02:29
Modulating Sensory Neuronal Signaling in a Mouse Model Using Near Infrared Light
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2025
Source: Zhang, L., et al. Near Infrared (NIr) Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium. J. Vis. Exp. (2015)This video demonstrates the process of applying NIr light to a shaved mouse scalp to activate vestibular neurons and stimulate superoxide dismutase-1 (SOD-1) production, supporting neuronal function. The procedure highlights NIr therapy as a neuroprotective strategy.
09:23
Inducing Targeted Mild Hyperthermia in Murine Tumor Models through Photothermal Conversion of Near-infrared Light by Intratumoral Gold Nanorods
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2025
This protocol presents techniques and methodology necessary for targeted hyperthermic therapy in solid tumor models. The approach leverages the photothermal conversion of near-infrared light by intratumorally injected gold nanorods to induce localized heating within the tumor microenvironment.
06:56
Near Infrared (NIr) Light Increases Expression of a Marker of Mitochondrial Function in the Mouse Vestibular Sensory Epithelium
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Cited by 4 •
2015
Mitochondrial dysfunction is a hallmark of cellular senescence. This paper uses non-invasive near-infrared (NIr) treatment to improve mitochondrial function in the aging mouse vestibular sensory epithelium.
10:26
Soft Lithographic Procedure for Producing Plastic Microfluidic Devices with View-ports Transparent to Visible and Infrared Light
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Cited by 1 •
2017
A protocol for the fabrication of plastic microfluidic devices with transparent view-ports for visible and infrared light imaging is described.
03:40
UV Irradiation: A Method to Integrate Extrachromosomal Arrays
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2023
Microinjection of transgenes usually results in C. elegans strains with large extrachromosomal arrays, which result in variable expression and transmission of the transgene. UV Irradiation can induce chromosomal integration of the array and more stable transgene transmission.
FAQs
Chromophores absorb selected wavelengths and convert the incoming light into biological effects. Depending on the experimental conditions, this energy can influence mitochondrial activity, generate reactive oxygen species, affect membrane integrity, or alter immune signaling. Consequently, changing the wavelength may change which cellular or microbial responses are observed, making spectral selection an important part of experimental design.
The outcome depends on how absorbed light energy is processed and on the exposure conditions. Photochemical effects may involve altered reactive oxygen species or signaling, whereas localized thermal effects reflect energy conversion into heat. Because both responses can occur, experiments should distinguish biological changes caused by light-dependent chemistry from those associated with temperature elevation.
Wavelength, intensity, exposure time, and temperature are central variables because they determine the amount and type of energy delivered to the sample. Small changes in these conditions can alter mitochondrial activity, reactive oxygen species, membrane integrity, or immune signaling. Controlling them carefully is therefore necessary when comparing cellular, microbial, or immune outcomes.
Near-infrared light irradiation uses non-ionizing electromagnetic radiation, so its effects arise from chromophore absorption and subsequent photochemical or localized thermal processes rather than ionization. This distinction helps frame its experimental use: investigators examine changes in cellular or microbial behavior, immune signaling, inflammation, or tissue recovery without treating the exposure as an ionizing-radiation process.
A practical workflow begins by selecting the wavelength and defining the intended intensity and exposure time. The sample is then exposed under controlled temperature conditions, followed by measurement of the relevant biological response, such as mitochondrial activity, reactive oxygen species, membrane integrity, or immune signaling. Keeping these parameters consistent enables meaningful comparison across samples and experiments.
The approach can support investigations of inflammation, host-pathogen interactions, antimicrobial treatment, and tissue recovery. Researchers may examine whether exposure changes host-cell signaling, microbial behavior, membrane integrity, or other responses relevant to infection biology. Its value lies in connecting controlled light conditions with measurable effects in immune and microbial systems, while avoiding unsupported conclusions about outcomes outside the tested conditions.