Murine Lung Immune Cells
Murine Lung Immune Cells - Related Videos
05:54
An In Vitro Method to Identify Innate and Adaptive Immune Cells Residing in Murine Lungs
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2025
This video demonstrates an in vitro technique to identify immune cells residing in murine lungs. After isolating target immune cells from murine lung tissue, they are surface-labeled using a cocktail of fluorophore-tagged antibodies against specific markers on innate and adaptive immune cells. Additionally, monocytes, macrophages, and dendritic cells are labeled using an intracellular marker. The various immune cell types are then identified using flow cytometry.
03:51
Preparing a Single-Cell Suspension of Immune Cells from Murine Brain Tissue
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2025
The video demonstrates the preparation of a single-cell suspension of immune cells from a murine brain tissue. It involves enzymatic digestion to release cells from the brain tissue, followed by density gradient centrifugation to isolate the immune cells based on their densities to obtain the single-cell suspension.
02:46
Intranasal Murine Herpesvirus Infection in Mice to Assess Host Immune Responses
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2026
Source: Zhang, J., et al. P. Dissecting Innate Immune Signaling in Viral Evasion of Cytokine Production. J. Vis. Exp. (2014)This video demonstrates intranasal delivery of murine herpesvirus in mice to induce a lung-specific immune response. Lung tissue is subsequently processed to obtain cytokine-containing supernatants for downstream assessment of host immune responses.
03:19
Isolation of Gingival Immune Cell Network from Murine Teeth Blocks
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2025
This video demonstrates the isolation procedure of the gingival immune cell network from a mouse model. The enzymatic digestion releases the gingival tissue from the attached teeth and bone, which is further mechanically digested, releasing immune cells from the gingiva. The isolated cells are ready for immunological experiments.
07:03
Bronchoalveolar Lavage of Murine Lungs to Analyze Inflammatory Cell Infiltration
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Cited by 155 •
2017
The health status of the lung is reflected by the type and number of immune cells that are present in the bronchioles of the lung. We describe a bronchoalveolar lavage technique that allows the isolation and study of nonadherent cells and soluble factors from the lower respiratory tract of mice.
FAQs
Pattern-recognition receptors detect pathogen-associated signals and initiate coordinated cellular responses. In murine lungs, this recognition can promote phagocytosis, antigen presentation, cytokine release, and recruitment of additional leukocytes. The resulting response helps connect early detection with broader immune activity, allowing researchers to examine how respiratory pathogens trigger defense mechanisms and how those mechanisms may also contribute to inflammation.
These populations support pulmonary defense through complementary activities rather than a single uniform response. Macrophages and neutrophils contribute to phagocytic defense, dendritic cells support antigen presentation, and lymphocytes participate in coordinated immune responses. Examining their relative contributions helps distinguish immediate cellular protection from processes that organize or sustain responses during infection and inflammation.
Inflammation can help control respiratory infection, yet excessive or poorly regulated activity may damage host tissues. Murine lung immune cells provide a way to study this balance by linking cytokine release, cellular recruitment, and pathogen recognition with the resulting immune outcome. This distinction is important when evaluating whether a response is protective, harmful, or influenced by an intervention.
Researchers can examine how pathogen-associated signals interact with lung leukocytes and how those cells coordinate phagocytosis, antigen presentation, cytokine release, and recruitment. Mouse models support controlled investigation of these host-pathogen interactions, including the comparison of immune responses under defined experimental conditions. Such studies help clarify mechanisms that may determine whether infection is controlled or accompanied by damaging inflammation.
Mice allow controlled genetic and experimental manipulation, making it possible to investigate how defined changes affect lung immune responses. Researchers can use this system to connect particular immune mechanisms with responses to respiratory pathogens, vaccines, or therapeutics. The model therefore supports mechanistic studies in which immune-cell behavior is examined in relation to a controlled infection or intervention.
Their responses can reveal how an intervention influences pathogen recognition, antigen presentation, cytokine production, cellular recruitment, and the balance between protection and inflammation. In mouse studies, these immune outcomes help researchers evaluate vaccines or therapeutics before interpreting their effects in the broader context of respiratory infection. The approach links cellular immune activity with experimental treatment performance.